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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27336 | 5' | -54.9 | NC_005857.1 | + | 49665 | 0.66 | 0.731992 |
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Target: 5'- aGCCCACGGCgGuACUGAugacgauuccaaucuGGAaucguugugcUGGCCGu -3' miRNA: 3'- -CGGGUGCUGgCcUGACU---------------UCU----------AUCGGU- -5' |
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| 27336 | 5' | -54.9 | NC_005857.1 | + | 21592 | 0.66 | 0.727734 |
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Target: 5'- cGCCgACaGcACCGGACUGAu--UGGCUc -3' miRNA: 3'- -CGGgUG-C-UGGCCUGACUucuAUCGGu -5' |
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| 27336 | 5' | -54.9 | NC_005857.1 | + | 2963 | 0.66 | 0.706227 |
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Target: 5'- cGCCgACGuACuCGGGCUGAAucUGGCg- -3' miRNA: 3'- -CGGgUGC-UG-GCCUGACUUcuAUCGgu -5' |
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| 27336 | 5' | -54.9 | NC_005857.1 | + | 17435 | 0.67 | 0.673443 |
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Target: 5'- gGCCagauaACGACCGGcguaaaugccGCUGAccucgaacgAGGUGGUCGa -3' miRNA: 3'- -CGGg----UGCUGGCC----------UGACU---------UCUAUCGGU- -5' |
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| 27336 | 5' | -54.9 | NC_005857.1 | + | 8857 | 0.67 | 0.651371 |
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Target: 5'- cGCCCugG-CUGGAgCUGAuuuucuGGcUGGCCGa -3' miRNA: 3'- -CGGGugCuGGCCU-GACU------UCuAUCGGU- -5' |
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| 27336 | 5' | -54.9 | NC_005857.1 | + | 17715 | 0.68 | 0.585047 |
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Target: 5'- aGUCCguggACGGCCuGGguGCaGggGAUGGCCAg -3' miRNA: 3'- -CGGG----UGCUGG-CC--UGaCuuCUAUCGGU- -5' |
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| 27336 | 5' | -54.9 | NC_005857.1 | + | 14239 | 0.69 | 0.530768 |
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Target: 5'- cGCCaUACGGCaCGcGCUGGcugAGGUGGCCAu -3' miRNA: 3'- -CGG-GUGCUG-GCcUGACU---UCUAUCGGU- -5' |
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| 27336 | 5' | -54.9 | NC_005857.1 | + | 6929 | 1.11 | 0.00079 |
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Target: 5'- gGCCCACGACCGGACUGAAGAUAGCCAa -3' miRNA: 3'- -CGGGUGCUGGCCUGACUUCUAUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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