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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27341 | 5' | -51.3 | NC_005857.1 | + | 27330 | 0.66 | 0.869531 |
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Target: 5'- aAUCACCAaccauuggcgacacuGACCCCGauGUAGgggCGUGGUg -3' miRNA: 3'- gUAGUGGU---------------CUGGGGU--CGUUa--GUAUCA- -5' |
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| 27341 | 5' | -51.3 | NC_005857.1 | + | 13177 | 0.66 | 0.857621 |
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Target: 5'- aCGUCAgCAGACCCUAcGCAggaaaagcGUCAgcuGUa -3' miRNA: 3'- -GUAGUgGUCUGGGGU-CGU--------UAGUau-CA- -5' |
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| 27341 | 5' | -51.3 | NC_005857.1 | + | 50229 | 0.66 | 0.848802 |
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Target: 5'- uGUCAUCAGGgCCagguCAGCAAUCGgAGUu -3' miRNA: 3'- gUAGUGGUCUgGG----GUCGUUAGUaUCA- -5' |
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| 27341 | 5' | -51.3 | NC_005857.1 | + | 36556 | 0.69 | 0.704369 |
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Target: 5'- gCAUUACCAgGACCCUGGCuAUCAg--- -3' miRNA: 3'- -GUAGUGGU-CUGGGGUCGuUAGUauca -5' |
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| 27341 | 5' | -51.3 | NC_005857.1 | + | 13975 | 1.08 | 0.002394 |
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Target: 5'- uCAUCACCAGACCCCAGCAAUCAUAGUg -3' miRNA: 3'- -GUAGUGGUCUGGGGUCGUUAGUAUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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