Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27342 | 3' | -52.7 | NC_005857.1 | + | 41391 | 0.66 | 0.835409 |
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Target: 5'- -gACGGCg--CUGCgugcaaaCACCGC-CCAGg -3' miRNA: 3'- caUGCUGuuaGACGa------GUGGCGaGGUC- -5' |
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| 27342 | 3' | -52.7 | NC_005857.1 | + | 9700 | 0.66 | 0.787047 |
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Target: 5'- aUAUaACGAUCUGCUCcugugcACuCGCUCCGu -3' miRNA: 3'- cAUGcUGUUAGACGAG------UG-GCGAGGUc -5' |
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| 27342 | 3' | -52.7 | NC_005857.1 | + | 33873 | 0.67 | 0.744142 |
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Target: 5'- uGUGCGGCAAUUUGCUUcgcugauAUCGCUgaaaCAGu -3' miRNA: 3'- -CAUGCUGUUAGACGAG-------UGGCGAg---GUC- -5' |
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| 27342 | 3' | -52.7 | NC_005857.1 | + | 17628 | 0.68 | 0.723521 |
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Target: 5'- uGUACuGCAGcucgguuuUCUGCguaUCGCCGCUCCc- -3' miRNA: 3'- -CAUGcUGUU--------AGACG---AGUGGCGAGGuc -5' |
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| 27342 | 3' | -52.7 | NC_005857.1 | + | 18644 | 0.68 | 0.701441 |
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Target: 5'- -aACGACAGUCgcaUGCUgACCGUcgcagagCCGGa -3' miRNA: 3'- caUGCUGUUAG---ACGAgUGGCGa------GGUC- -5' |
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| 27342 | 3' | -52.7 | NC_005857.1 | + | 10620 | 0.71 | 0.522088 |
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Target: 5'- --uCGACA-UCUGCcgguUCGCCGCUgCCAGu -3' miRNA: 3'- cauGCUGUuAGACG----AGUGGCGA-GGUC- -5' |
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| 27342 | 3' | -52.7 | NC_005857.1 | + | 1883 | 0.73 | 0.418734 |
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Target: 5'- -cGCGAUAGUCUGcCUCugCGCcauccgucgcuUCCAGc -3' miRNA: 3'- caUGCUGUUAGAC-GAGugGCG-----------AGGUC- -5' |
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| 27342 | 3' | -52.7 | NC_005857.1 | + | 16478 | 1.08 | 0.00187 |
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Target: 5'- uGUACGACAAUCUGCUCACCGCUCCAGc -3' miRNA: 3'- -CAUGCUGUUAGACGAGUGGCGAGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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