Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27342 | 5' | -51.1 | NC_005857.1 | + | 34515 | 0.66 | 0.919043 |
Target: 5'- -aCCGGAGGccugGGCGGaGAAcgCGCu -3' miRNA: 3'- ggGGCCUUUua--CCGCCgUUUuaGCGc -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 10299 | 0.66 | 0.919043 |
Target: 5'- gCCUggCGGGAAAgccaGGCGGCGGuaaaaGCGa -3' miRNA: 3'- -GGG--GCCUUUUa---CCGCCGUUuuag-CGC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 33400 | 0.66 | 0.91265 |
Target: 5'- gCCUGGAGGAUGaGCguaGGCAuuuugccuaucAAGUCGuCGa -3' miRNA: 3'- gGGGCCUUUUAC-CG---CCGU-----------UUUAGC-GC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 7108 | 0.66 | 0.91265 |
Target: 5'- -aCCGGGGAGgcagacgGGCGGCAuGAUagaacugGCGa -3' miRNA: 3'- ggGGCCUUUUa------CCGCCGUuUUAg------CGC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 4434 | 0.66 | 0.905969 |
Target: 5'- cCCCUGGAAcAUGGCcuguGCuucuUCGCu -3' miRNA: 3'- -GGGGCCUUuUACCGc---CGuuuuAGCGc -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 48528 | 0.66 | 0.891753 |
Target: 5'- gCgCCGGAGGAcguuugccagcUGGgGaGCAucaGAAUCGCGu -3' miRNA: 3'- -GgGGCCUUUU-----------ACCgC-CGU---UUUAGCGC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 31750 | 0.66 | 0.891753 |
Target: 5'- gCgCCGGuGAAccauuggacUGGCGGCAAAAggaagaauuaCGCGg -3' miRNA: 3'- -GgGGCCuUUU---------ACCGCCGUUUUa---------GCGC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 9034 | 0.67 | 0.874037 |
Target: 5'- aUCCCGGAcgaagauggaagagGAAUGcGCGGCAGucauGAggGCa -3' miRNA: 3'- -GGGGCCU--------------UUUAC-CGCCGUU----UUagCGc -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 1794 | 0.68 | 0.805166 |
Target: 5'- gCgCUGGAAgcgacgGAUGGCgcagaGGCAGAcuAUCGCGa -3' miRNA: 3'- -GgGGCCUU------UUACCG-----CCGUUU--UAGCGC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 20730 | 0.69 | 0.785233 |
Target: 5'- --aCGGAAAGUGGUcugGGCGAg--CGCGg -3' miRNA: 3'- gggGCCUUUUACCG---CCGUUuuaGCGC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 24356 | 0.69 | 0.785233 |
Target: 5'- --aCGGAAAGUGGUcugGGCGAg--CGCGg -3' miRNA: 3'- gggGCCUUUUACCG---CCGUUuuaGCGC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 12627 | 0.69 | 0.77501 |
Target: 5'- uUCCUGGAucgucAUGGCGGCAcaAAGUCu-- -3' miRNA: 3'- -GGGGCCUuu---UACCGCCGU--UUUAGcgc -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 5755 | 0.71 | 0.670748 |
Target: 5'- aCCUCGGAGAG-GGCGGgAAAGagucagaaauuuacuUCGCu -3' miRNA: 3'- -GGGGCCUUUUaCCGCCgUUUU---------------AGCGc -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 48742 | 0.71 | 0.651613 |
Target: 5'- cCUCUGGAgauuuaccagcgaaGAGUGGUGcaGCAGAAUUGCGa -3' miRNA: 3'- -GGGGCCU--------------UUUACCGC--CGUUUUAGCGC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 17532 | 0.72 | 0.62113 |
Target: 5'- uUCCCGGAGGGgaGCGGCGAua-CGCa -3' miRNA: 3'- -GGGGCCUUUUacCGCCGUUuuaGCGc -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 4368 | 0.73 | 0.553933 |
Target: 5'- aCCCCGGAAGAUcGUGGUAAAcGUCaGCc -3' miRNA: 3'- -GGGGCCUUUUAcCGCCGUUU-UAG-CGc -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 47990 | 0.73 | 0.542912 |
Target: 5'- gCCCGcaauGAAGAUGaGCGGCAGg--CGCGg -3' miRNA: 3'- gGGGC----CUUUUAC-CGCCGUUuuaGCGC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 8651 | 0.74 | 0.489047 |
Target: 5'- uCCCCGGuca--GGCGGCGGAuauagaccGUUGCGc -3' miRNA: 3'- -GGGGCCuuuuaCCGCCGUUU--------UAGCGC- -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 43056 | 0.76 | 0.408596 |
Target: 5'- aCCCCGGAAAGccagcaGGCGGCAGGuuauacCGCc -3' miRNA: 3'- -GGGGCCUUUUa-----CCGCCGUUUua----GCGc -5' |
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27342 | 5' | -51.1 | NC_005857.1 | + | 16443 | 1.11 | 0.002089 |
Target: 5'- cCCCCGGAAAAUGGCGGCAAAAUCGCGc -3' miRNA: 3'- -GGGGCCUUUUACCGCCGUUUUAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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