Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27343 | 3' | -58.2 | NC_005857.1 | + | 37582 | 0.66 | 0.564068 |
Target: 5'- aGCguaucGACCAGCgcggCGGGAaauuccaGAccaGCGCCa -3' miRNA: 3'- aCGa----CUGGUCGag--GCCCUg------CU---UGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 10837 | 0.66 | 0.564068 |
Target: 5'- aGCUGAUgaaAGagaUUCGGGACGcuGACaGCCg -3' miRNA: 3'- aCGACUGg--UCg--AGGCCCUGC--UUG-CGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 5972 | 0.66 | 0.553456 |
Target: 5'- cGCgGAUCAGCgacugguuaCGGGA-GAACGCg -3' miRNA: 3'- aCGaCUGGUCGag-------GCCCUgCUUGCGg -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 2923 | 0.66 | 0.536605 |
Target: 5'- cGCUGGauGGCUauaUCGGuuccucucccauucaGACGAACGCCg -3' miRNA: 3'- aCGACUggUCGA---GGCC---------------CUGCUUGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 13708 | 0.66 | 0.522009 |
Target: 5'- cGCUGGCCAgGUUCCugcaGGGAaaauAGCGUCc -3' miRNA: 3'- aCGACUGGU-CGAGG----CCCUgc--UUGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 1065 | 0.67 | 0.475233 |
Target: 5'- gGCUGGCCAucuGCcuggCUGGGGUGAagaccuuuuucgccuGCGCCa -3' miRNA: 3'- aCGACUGGU---CGa---GGCCCUGCU---------------UGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 46759 | 0.67 | 0.471263 |
Target: 5'- gGCUGGucuuacucaugUUAGCcgCCGGGGugauauCGAGCGCCc -3' miRNA: 3'- aCGACU-----------GGUCGa-GGCCCU------GCUUGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 13237 | 0.67 | 0.461411 |
Target: 5'- cUGCUGACguGUuucccUCCGGGAaguuuuGCGCa -3' miRNA: 3'- -ACGACUGguCG-----AGGCCCUgcu---UGCGg -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 34967 | 0.67 | 0.461411 |
Target: 5'- -aCUGACCGGCgaggCCGacAUGGAUGCCc -3' miRNA: 3'- acGACUGGUCGa---GGCccUGCUUGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 30663 | 0.68 | 0.442041 |
Target: 5'- aGCUGgauagcaugauGCCAGauuuugCCaGGGGCG-ACGCCg -3' miRNA: 3'- aCGAC-----------UGGUCga----GG-CCCUGCuUGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 30179 | 0.68 | 0.442041 |
Target: 5'- gGC-GACCAGauagCgCGgcaaGGGCGAACGCCa -3' miRNA: 3'- aCGaCUGGUCga--G-GC----CCUGCUUGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 6153 | 0.68 | 0.432531 |
Target: 5'- aUGC-GGCCAGCaguaauaCCGGuGACGccacagauGGCGCCg -3' miRNA: 3'- -ACGaCUGGUCGa------GGCC-CUGC--------UUGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 37630 | 0.68 | 0.42314 |
Target: 5'- cGCUGGCCca--CCuGGACGGGCGCa -3' miRNA: 3'- aCGACUGGucgaGGcCCUGCUUGCGg -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 22265 | 0.69 | 0.386837 |
Target: 5'- cGCUGAaugCAGCcgUUCGGGcCGGaaACGCCu -3' miRNA: 3'- aCGACUg--GUCG--AGGCCCuGCU--UGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 8881 | 0.69 | 0.36099 |
Target: 5'- gGCUGGCCGaCcgCUGGGGCGAGC-CUg -3' miRNA: 3'- aCGACUGGUcGa-GGCCCUGCUUGcGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 20080 | 0.7 | 0.328442 |
Target: 5'- cGCUGACCAGC-CaGGGGCaGGCGaUCa -3' miRNA: 3'- aCGACUGGUCGaGgCCCUGcUUGC-GG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 23707 | 0.7 | 0.328442 |
Target: 5'- cGCUGACCAGC-CaGGGGCaGGCGaUCa -3' miRNA: 3'- aCGACUGGUCGaGgCCCUGcUUGC-GG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 20110 | 0.72 | 0.256768 |
Target: 5'- aGCUGAaUAGCgacCUGGccacGACGAACGCCa -3' miRNA: 3'- aCGACUgGUCGa--GGCC----CUGCUUGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 23737 | 0.72 | 0.256768 |
Target: 5'- aGCUGAaUAGCgacCUGGccacGACGAACGCCa -3' miRNA: 3'- aCGACUgGUCGa--GGCC----CUGCUUGCGG- -5' |
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27343 | 3' | -58.2 | NC_005857.1 | + | 23002 | 0.73 | 0.214596 |
Target: 5'- aGCUGACCAGUgacCUGucGACGAccaACGCCa -3' miRNA: 3'- aCGACUGGUCGa--GGCc-CUGCU---UGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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