Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27347 | 3' | -45.4 | NC_005857.1 | + | 21965 | 0.66 | 0.995981 |
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Target: 5'- uUUUUAuuCACA-UUCCCAuuGGUGGUc -3' miRNA: 3'- uAAAGUuuGUGUaGAGGGUu-UCACCG- -5' |
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| 27347 | 3' | -45.4 | NC_005857.1 | + | 27430 | 0.66 | 0.995981 |
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Target: 5'- -cUUCAuuauUACucCUCCCAAcGUGGCu -3' miRNA: 3'- uaAAGUuu--GUGuaGAGGGUUuCACCG- -5' |
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| 27347 | 3' | -45.4 | NC_005857.1 | + | 6817 | 0.66 | 0.995981 |
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Target: 5'- gGUUUCcAGC-CAUUgCCCGGuGUGGCc -3' miRNA: 3'- -UAAAGuUUGuGUAGaGGGUUuCACCG- -5' |
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| 27347 | 3' | -45.4 | NC_005857.1 | + | 34545 | 0.67 | 0.992093 |
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Target: 5'- ---cCAGGCACuggagGUCaUCCC--GGUGGCg -3' miRNA: 3'- uaaaGUUUGUG-----UAG-AGGGuuUCACCG- -5' |
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| 27347 | 3' | -45.4 | NC_005857.1 | + | 36639 | 0.67 | 0.990617 |
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Target: 5'- cGUUUCuGGGCGCAccgugucUCUCUCAuuGGUGGUc -3' miRNA: 3'- -UAAAG-UUUGUGU-------AGAGGGUu-UCACCG- -5' |
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| 27347 | 3' | -45.4 | NC_005857.1 | + | 37705 | 0.67 | 0.989255 |
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Target: 5'- uUUUCAGcugGCGCAUgCUCCCA----GGCa -3' miRNA: 3'- uAAAGUU---UGUGUA-GAGGGUuucaCCG- -5' |
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| 27347 | 3' | -45.4 | NC_005857.1 | + | 49645 | 0.71 | 0.931824 |
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Target: 5'- --gUCGAcCACAUCauUCCCAAAGcccacGGCg -3' miRNA: 3'- uaaAGUUuGUGUAG--AGGGUUUCa----CCG- -5' |
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| 27347 | 3' | -45.4 | NC_005857.1 | + | 11123 | 0.73 | 0.846469 |
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Target: 5'- cUUUCAGAU-CAUCUaCCAGGGUGGUu -3' miRNA: 3'- uAAAGUUUGuGUAGAgGGUUUCACCG- -5' |
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| 27347 | 3' | -45.4 | NC_005857.1 | + | 13094 | 0.78 | 0.586402 |
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Target: 5'- --gUCGGuuGCGCAUCgcucCCCGAGGUGGUg -3' miRNA: 3'- uaaAGUU--UGUGUAGa---GGGUUUCACCG- -5' |
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| 27347 | 3' | -45.4 | NC_005857.1 | + | 22539 | 1.13 | 0.005173 |
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Target: 5'- cAUUUCAAACACAUCUCCCAAAGUGGCa -3' miRNA: 3'- -UAAAGUUUGUGUAGAGGGUUUCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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