Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27347 | 5' | -52.3 | NC_005857.1 | + | 18978 | 0.67 | 0.806302 |
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Target: 5'- cCUGUUGUuUCAGGA-GAUGAaUGCGCg -3' miRNA: 3'- -GGCGGUAcAGUCCUaCUACUaGCGCG- -5' |
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| 27347 | 5' | -52.3 | NC_005857.1 | + | 4368 | 0.67 | 0.79654 |
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Target: 5'- -gGCCAUGUucCAGGG-GAUGAUUGaCGa -3' miRNA: 3'- ggCGGUACA--GUCCUaCUACUAGC-GCg -5' |
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| 27347 | 5' | -52.3 | NC_005857.1 | + | 17206 | 0.68 | 0.776495 |
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Target: 5'- aCGCUua-UCGGGcgcuggucGAUGAUCGCGCc -3' miRNA: 3'- gGCGGuacAGUCCua------CUACUAGCGCG- -5' |
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| 27347 | 5' | -52.3 | NC_005857.1 | + | 49727 | 0.73 | 0.482716 |
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Target: 5'- aCCGCCGUGggcuUUGGGaAUGAugUGGUCGaCGCu -3' miRNA: 3'- -GGCGGUAC----AGUCC-UACU--ACUAGC-GCG- -5' |
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| 27347 | 5' | -52.3 | NC_005857.1 | + | 17566 | 0.79 | 0.208941 |
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Target: 5'- aCGCCAUGcagCAGGGaagUGGUGGUCaGCGCc -3' miRNA: 3'- gGCGGUACa--GUCCU---ACUACUAG-CGCG- -5' |
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| 27347 | 5' | -52.3 | NC_005857.1 | + | 22505 | 1.13 | 0.001076 |
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Target: 5'- gCCGCCAUGUCAGGAUGAUGAUCGCGCc -3' miRNA: 3'- -GGCGGUACAGUCCUACUACUAGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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