miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27348 3' -51 NC_005857.1 + 29707 0.66 0.891909
Target:  5'- ----aUGCUGUCuaAUUgaUGCCGCAg- -3'
miRNA:   3'- cgaaaACGACAGcgUAG--ACGGCGUag -5'
27348 3' -51 NC_005857.1 + 26156 0.66 0.876218
Target:  5'- --aUUUGCUGcUCGCAUaaaauacguagCUGCCGgAUg -3'
miRNA:   3'- cgaAAACGAC-AGCGUA-----------GACGGCgUAg -5'
27348 3' -51 NC_005857.1 + 36880 0.66 0.867952
Target:  5'- cGCgucgGCUGaaUCGCAaaUGCCGCGc- -3'
miRNA:   3'- -CGaaaaCGAC--AGCGUagACGGCGUag -5'
27348 3' -51 NC_005857.1 + 8300 0.69 0.749454
Target:  5'- -----aGCUGaauggucUCGCGagccUCUGCCGCAUUa -3'
miRNA:   3'- cgaaaaCGAC-------AGCGU----AGACGGCGUAG- -5'
27348 3' -51 NC_005857.1 + 18966 0.7 0.683311
Target:  5'- gGCUc--GCUuauUCGCuUCUGCCGUAUCg -3'
miRNA:   3'- -CGAaaaCGAc--AGCGuAGACGGCGUAG- -5'
27348 3' -51 NC_005857.1 + 22850 1.11 0.001467
Target:  5'- gGCUUUUGCUGUCGCAUCUGCCGCAUCc -3'
miRNA:   3'- -CGAAAACGACAGCGUAGACGGCGUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.