miRNA display CGI


Results 1 - 15 of 15 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27351 3' -58.6 NC_005857.1 + 23622 0.66 0.542905
Target:  5'- aCGGCGuggagcgaagcGCCGGAggacaacgccAGCGCGGC---GGCa -3'
miRNA:   3'- -GCCGC-----------UGGUCUa---------UCGCGCCGuucCCG- -5'
27351 3' -58.6 NC_005857.1 + 19995 0.66 0.542905
Target:  5'- aCGGCGuggagcgaagcGCCGGAggacaacgccAGCGCGGC---GGCa -3'
miRNA:   3'- -GCCGC-----------UGGUCUa---------UCGCGCCGuucCCG- -5'
27351 3' -58.6 NC_005857.1 + 23082 0.66 0.522009
Target:  5'- aCGGUGACgCAGc-AGgGgGGCAcgcuguccacccAGGGCa -3'
miRNA:   3'- -GCCGCUG-GUCuaUCgCgCCGU------------UCCCG- -5'
27351 3' -58.6 NC_005857.1 + 49681 0.66 0.522009
Target:  5'- gCGGCGAUaccuUGGCGuCGGCAGuucuGGCa -3'
miRNA:   3'- -GCCGCUGgucuAUCGC-GCCGUUc---CCG- -5'
27351 3' -58.6 NC_005857.1 + 51022 0.66 0.522009
Target:  5'- uGGCaGCCAGAUGGCGaaaucAGGGUu -3'
miRNA:   3'- gCCGcUGGUCUAUCGCgccguUCCCG- -5'
27351 3' -58.6 NC_005857.1 + 3454 0.67 0.48122
Target:  5'- uGGCGACCAGAaaucgcguUAugagucauauGCGCuuGGCcgccaguGGGGCu -3'
miRNA:   3'- gCCGCUGGUCU--------AU----------CGCG--CCGu------UCCCG- -5'
27351 3' -58.6 NC_005857.1 + 16264 0.67 0.471263
Target:  5'- -uGCGAUCAGAUGGUccccGaUGGUAAGGGa -3'
miRNA:   3'- gcCGCUGGUCUAUCG----C-GCCGUUCCCg -5'
27351 3' -58.6 NC_005857.1 + 9037 0.67 0.45167
Target:  5'- cCGGaCGAagauggaAGAggaauGCGCGGCAgucaugAGGGCa -3'
miRNA:   3'- -GCC-GCUgg-----UCUau---CGCGCCGU------UCCCG- -5'
27351 3' -58.6 NC_005857.1 + 6171 0.68 0.404732
Target:  5'- cCGGUGACgccaCAGAUGGCGCcgacGGUAGuGGUg -3'
miRNA:   3'- -GCCGCUG----GUCUAUCGCG----CCGUUcCCG- -5'
27351 3' -58.6 NC_005857.1 + 23194 0.69 0.368617
Target:  5'- gCGGCcuGGCCAGAUauggcaaugugAGCGCggaaaaauacaauGGCAacGGGGUg -3'
miRNA:   3'- -GCCG--CUGGUCUA-----------UCGCG-------------CCGU--UCCCG- -5'
27351 3' -58.6 NC_005857.1 + 47901 0.69 0.328442
Target:  5'- cCGuGCuGCCuugcuGAUAGCGuCGGCAuugcGGGCa -3'
miRNA:   3'- -GC-CGcUGGu----CUAUCGC-GCCGUu---CCCG- -5'
27351 3' -58.6 NC_005857.1 + 50531 0.7 0.298115
Target:  5'- gCGGauaGACCgcagcagccgaaGGGUGGCGauaGCAGGGGCg -3'
miRNA:   3'- -GCCg--CUGG------------UCUAUCGCgc-CGUUCCCG- -5'
27351 3' -58.6 NC_005857.1 + 27275 0.72 0.214596
Target:  5'- uGGCGACa--AUGGUGUGGCu-GGGCa -3'
miRNA:   3'- gCCGCUGgucUAUCGCGCCGuuCCCG- -5'
27351 3' -58.6 NC_005857.1 + 12203 0.74 0.178506
Target:  5'- cCGGUGACgGcucgcugggGGUAcGCGCGGuCGGGGGCg -3'
miRNA:   3'- -GCCGCUGgU---------CUAU-CGCGCC-GUUCCCG- -5'
27351 3' -58.6 NC_005857.1 + 30181 1.12 0.00028
Target:  5'- uCGGCGACCAGAUAGCGCGGCAAGGGCg -3'
miRNA:   3'- -GCCGCUGGUCUAUCGCGCCGUUCCCG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.