Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27352 | 5' | -55.4 | NC_005857.1 | + | 15440 | 0.66 | 0.626498 |
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Target: 5'- cAUCCGGCUgcuccGuCGGCGUgCGCU-GGCu -3' miRNA: 3'- -UAGGUCGAaaa--C-GUCGCG-GCGAuCCG- -5' |
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| 27352 | 5' | -55.4 | NC_005857.1 | + | 6864 | 0.67 | 0.547974 |
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Target: 5'- aGUCCGGUc---GUGG-GCCGUUGGGCg -3' miRNA: 3'- -UAGGUCGaaaaCGUCgCGGCGAUCCG- -5' |
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| 27352 | 5' | -55.4 | NC_005857.1 | + | 16874 | 0.67 | 0.54687 |
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Target: 5'- -aCCGGC---UGCAGCugaaccuGCCGUcAGGCa -3' miRNA: 3'- uaGGUCGaaaACGUCG-------CGGCGaUCCG- -5' |
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| 27352 | 5' | -55.4 | NC_005857.1 | + | 5601 | 0.68 | 0.493789 |
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Target: 5'- uAUCCAGauuaacUGCAGCgucuGCCGCccaAGGCa -3' miRNA: 3'- -UAGGUCgaaa--ACGUCG----CGGCGa--UCCG- -5' |
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| 27352 | 5' | -55.4 | NC_005857.1 | + | 19505 | 0.72 | 0.277718 |
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Target: 5'- uUCUgcaGGC-UUUGCAGCGCCGU--GGCa -3' miRNA: 3'- uAGG---UCGaAAACGUCGCGGCGauCCG- -5' |
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| 27352 | 5' | -55.4 | NC_005857.1 | + | 23131 | 0.72 | 0.277718 |
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Target: 5'- uUCUgcaGGC-UUUGCAGCGCCGU--GGCa -3' miRNA: 3'- uAGG---UCGaAAACGUCGCGGCGauCCG- -5' |
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| 27352 | 5' | -55.4 | NC_005857.1 | + | 4065 | 0.74 | 0.218586 |
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Target: 5'- uUCCGGCg---GCGGCGCaGCUguGGGCu -3' miRNA: 3'- uAGGUCGaaaaCGUCGCGgCGA--UCCG- -5' |
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| 27352 | 5' | -55.4 | NC_005857.1 | + | 30721 | 1.1 | 0.000524 |
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Target: 5'- uAUCCAGCUUUUGCAGCGCCGCUAGGCg -3' miRNA: 3'- -UAGGUCGAAAACGUCGCGGCGAUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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