Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27356 | 5' | -55.2 | NC_005857.1 | + | 23918 | 0.66 | 0.662529 |
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Target: 5'- gGACAgcaGCgCGGUgaacacgcugacgaACCGGGCAGCCa- -3' miRNA: 3'- aCUGUa--CGgGCCA--------------UGGUUUGUCGGgc -5' |
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| 27356 | 5' | -55.2 | NC_005857.1 | + | 40690 | 0.67 | 0.654775 |
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Target: 5'- -----aGCCUGGUgaaGCCGAGCcGCCCu -3' miRNA: 3'- acuguaCGGGCCA---UGGUUUGuCGGGc -5' |
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| 27356 | 5' | -55.2 | NC_005857.1 | + | 17695 | 0.68 | 0.588242 |
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Target: 5'- cUGAUAgguuuUGCCCGGauacGCCAaucaGACAGCgCCa -3' miRNA: 3'- -ACUGU-----ACGGGCCa---UGGU----UUGUCG-GGc -5' |
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| 27356 | 5' | -55.2 | NC_005857.1 | + | 4548 | 0.68 | 0.566265 |
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Target: 5'- -aACAgcGCCUGG-ACCGAaauACAGCCCa -3' miRNA: 3'- acUGUa-CGGGCCaUGGUU---UGUCGGGc -5' |
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| 27356 | 5' | -55.2 | NC_005857.1 | + | 43789 | 0.69 | 0.48111 |
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Target: 5'- cUGAauUGUCCGGUACUuaugcgcuCGGCCCGu -3' miRNA: 3'- -ACUguACGGGCCAUGGuuu-----GUCGGGC- -5' |
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| 27356 | 5' | -55.2 | NC_005857.1 | + | 16816 | 0.7 | 0.460772 |
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Target: 5'- aUGAUGUaGCCCGGcugcgggauuuUACCGucCAGCCCc -3' miRNA: 3'- -ACUGUA-CGGGCC-----------AUGGUuuGUCGGGc -5' |
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| 27356 | 5' | -55.2 | NC_005857.1 | + | 19741 | 0.78 | 0.155147 |
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Target: 5'- gGGCAgaagGUCCGGaGCCAuuucuACAGCCCGa -3' miRNA: 3'- aCUGUa---CGGGCCaUGGUu----UGUCGGGC- -5' |
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| 27356 | 5' | -55.2 | NC_005857.1 | + | 23367 | 0.78 | 0.155147 |
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Target: 5'- gGGCAgaagGUCCGGaGCCAuuucuACAGCCCGa -3' miRNA: 3'- aCUGUa---CGGGCCaUGGUu----UGUCGGGC- -5' |
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| 27356 | 5' | -55.2 | NC_005857.1 | + | 36992 | 1.09 | 0.000855 |
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Target: 5'- cUGACAUGCCCGGUACCAAACAGCCCGa -3' miRNA: 3'- -ACUGUACGGGCCAUGGUUUGUCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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