Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27358 | 5' | -54.4 | NC_005857.1 | + | 37397 | 0.67 | 0.729622 |
Target: 5'- ------cGCCGGUGG-CGAaaccUUCCACUa -3' miRNA: 3'- ccgagaaCGGCCACCuGCU----AAGGUGG- -5' |
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27358 | 5' | -54.4 | NC_005857.1 | + | 27782 | 0.67 | 0.686584 |
Target: 5'- aGGCgguuUCgaGCCGGUGGuACGAcugCUGCUa -3' miRNA: 3'- -CCG----AGaaCGGCCACC-UGCUaa-GGUGG- -5' |
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27358 | 5' | -54.4 | NC_005857.1 | + | 3502 | 0.68 | 0.642705 |
Target: 5'- gGGCUggUUGUCGGUuaACGAUaUCCGCCg -3' miRNA: 3'- -CCGAg-AACGGCCAccUGCUA-AGGUGG- -5' |
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27358 | 5' | -54.4 | NC_005857.1 | + | 2004 | 1.15 | 0.000466 |
Target: 5'- cGGCUCUUGCCGGUGGACGAUUCCACCa -3' miRNA: 3'- -CCGAGAACGGCCACCUGCUAAGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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