Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27359 | 5' | -55.8 | NC_005857.1 | + | 32622 | 0.66 | 0.578018 |
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Target: 5'- gGCAGCUuucgGGCUGGCCGguagcugauugggcCGGUAUUc-- -3' miRNA: 3'- gCGUCGG----UCGAUCGGC--------------GCCGUAAuuu -5' |
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| 27359 | 5' | -55.8 | NC_005857.1 | + | 13421 | 0.67 | 0.536968 |
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Target: 5'- uCGCAGCCAucGCc-GCUGCGGUAc---- -3' miRNA: 3'- -GCGUCGGU--CGauCGGCGCCGUaauuu -5' |
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| 27359 | 5' | -55.8 | NC_005857.1 | + | 35099 | 0.67 | 0.504443 |
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Target: 5'- uCGCuGCCAGCgaccGGCCacGCGGUAa---- -3' miRNA: 3'- -GCGuCGGUCGa---UCGG--CGCCGUaauuu -5' |
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| 27359 | 5' | -55.8 | NC_005857.1 | + | 6411 | 0.68 | 0.452267 |
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Target: 5'- aCGCGGaagAGCUGGUuucuCGCGGCAUUGc- -3' miRNA: 3'- -GCGUCgg-UCGAUCG----GCGCCGUAAUuu -5' |
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| 27359 | 5' | -55.8 | NC_005857.1 | + | 22758 | 0.69 | 0.412725 |
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Target: 5'- uGCGGCCAGCgcucaGGauGCGGCAg---- -3' miRNA: 3'- gCGUCGGUCGa----UCggCGCCGUaauuu -5' |
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| 27359 | 5' | -55.8 | NC_005857.1 | + | 17539 | 0.69 | 0.403178 |
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Target: 5'- aGC-GCCAGCgguGCCGaCGGCAa---- -3' miRNA: 3'- gCGuCGGUCGau-CGGC-GCCGUaauuu -5' |
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| 27359 | 5' | -55.8 | NC_005857.1 | + | 21859 | 0.69 | 0.393771 |
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Target: 5'- gGCuGCCGauggcGUUAGCUGCGGCAUc--- -3' miRNA: 3'- gCGuCGGU-----CGAUCGGCGCCGUAauuu -5' |
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| 27359 | 5' | -55.8 | NC_005857.1 | + | 8809 | 0.7 | 0.348928 |
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Target: 5'- aGCAGCuCuGC-AGCCGUGGguUUAAAc -3' miRNA: 3'- gCGUCG-GuCGaUCGGCGCCguAAUUU- -5' |
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| 27359 | 5' | -55.8 | NC_005857.1 | + | 4072 | 0.78 | 0.111187 |
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Target: 5'- cCGCGGUCAGCUGGCUGCGGa------ -3' miRNA: 3'- -GCGUCGGUCGAUCGGCGCCguaauuu -5' |
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| 27359 | 5' | -55.8 | NC_005857.1 | + | 4002 | 0.94 | 0.007832 |
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Target: 5'- cCGCAGCCAGCUGaCCGCGGCAUUAAAc -3' miRNA: 3'- -GCGUCGGUCGAUcGGCGCCGUAAUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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