Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27361 | 3' | -53.4 | NC_005857.1 | + | 17724 | 0.66 | 0.77511 |
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Target: 5'- aCGGccugGGUGCAGGGGAUGg---- -3' miRNA: 3'- gGCCuuuaCCGCGUUCCCUACgacuu -5' |
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| 27361 | 3' | -53.4 | NC_005857.1 | + | 9070 | 0.67 | 0.688375 |
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Target: 5'- uUCGGGAGgaGGCGCAuuuucaucgccAGGGcgGCgGAAa -3' miRNA: 3'- -GGCCUUUa-CCGCGU-----------UCCCuaCGaCUU- -5' |
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| 27361 | 3' | -53.4 | NC_005857.1 | + | 7717 | 0.69 | 0.586629 |
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Target: 5'- aCCcGAAA-GGCGCAAGGGaAUGCg--- -3' miRNA: 3'- -GGcCUUUaCCGCGUUCCC-UACGacuu -5' |
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| 27361 | 3' | -53.4 | NC_005857.1 | + | 11263 | 0.69 | 0.560857 |
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Target: 5'- gCGGGAagcagcgaagaaaaAUGGCGCcAGcGAUGCUGAc -3' miRNA: 3'- gGCCUU--------------UACCGCGuUCcCUACGACUu -5' |
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| 27361 | 3' | -53.4 | NC_005857.1 | + | 6170 | 0.71 | 0.487951 |
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Target: 5'- aCCGGugacgccacAGAUGGCGCcgacgguaguGGuGAUGCUGAAg -3' miRNA: 3'- -GGCC---------UUUACCGCGuu--------CC-CUACGACUU- -5' |
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| 27361 | 3' | -53.4 | NC_005857.1 | + | 5690 | 1.08 | 0.001319 |
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Target: 5'- cCCGGAAAUGGCGCAAGGGAUGCUGAAg -3' miRNA: 3'- -GGCCUUUACCGCGUUCCCUACGACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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