Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27362 | 5' | -55.3 | NC_005857.1 | + | 38166 | 0.66 | 0.680666 |
Target: 5'- ----aGUUGAacCCGCGCGGCGGGGa -3' miRNA: 3'- ucuacCAAUUgcGGCGCGUCGCCUCg -5' |
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27362 | 5' | -55.3 | NC_005857.1 | + | 4089 | 0.66 | 0.658605 |
Target: 5'- uGAUGGaucguUUAAUGCCGCGguCAGCuGGcuGCg -3' miRNA: 3'- uCUACC-----AAUUGCGGCGC--GUCG-CCu-CG- -5' |
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27362 | 5' | -55.3 | NC_005857.1 | + | 48200 | 0.68 | 0.537611 |
Target: 5'- ---cGGUUAcauUGCCGCGCuugaAGCGG-GCu -3' miRNA: 3'- ucuaCCAAUu--GCGGCGCG----UCGCCuCG- -5' |
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27362 | 5' | -55.3 | NC_005857.1 | + | 17547 | 0.69 | 0.516261 |
Target: 5'- uGGUGGUcAGCGCCaGCGguGCcGAcgGCa -3' miRNA: 3'- uCUACCAaUUGCGG-CGCguCGcCU--CG- -5' |
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27362 | 5' | -55.3 | NC_005857.1 | + | 31494 | 0.7 | 0.425133 |
Target: 5'- gAGGUGGUUcGCGggGCGCGuCGGAGUg -3' miRNA: 3'- -UCUACCAAuUGCggCGCGUcGCCUCG- -5' |
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27362 | 5' | -55.3 | NC_005857.1 | + | 48185 | 0.7 | 0.425133 |
Target: 5'- ---cGGUUuuucCGCUGCGCA-CGGAGCc -3' miRNA: 3'- ucuaCCAAuu--GCGGCGCGUcGCCUCG- -5' |
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27362 | 5' | -55.3 | NC_005857.1 | + | 15001 | 0.73 | 0.317553 |
Target: 5'- aGGAUugGGUaAAUGCUGCGUaugguauacaagcaGGCGGAGCc -3' miRNA: 3'- -UCUA--CCAaUUGCGGCGCG--------------UCGCCUCG- -5' |
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27362 | 5' | -55.3 | NC_005857.1 | + | 20014 | 0.73 | 0.282217 |
Target: 5'- cGGA-GGacAACGCCaGCGCGGCGGcAGUg -3' miRNA: 3'- -UCUaCCaaUUGCGG-CGCGUCGCC-UCG- -5' |
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27362 | 5' | -55.3 | NC_005857.1 | + | 23641 | 0.73 | 0.282217 |
Target: 5'- cGGA-GGacAACGCCaGCGCGGCGGcAGUg -3' miRNA: 3'- -UCUaCCaaUUGCGG-CGCGUCGCC-UCG- -5' |
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27362 | 5' | -55.3 | NC_005857.1 | + | 6576 | 1.12 | 0.000507 |
Target: 5'- cAGAUGGUUAACGCCGCGCAGCGGAGCa -3' miRNA: 3'- -UCUACCAAUUGCGGCGCGUCGCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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