Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27363 | 3' | -55.1 | NC_005857.1 | + | 46549 | 0.67 | 0.665312 |
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Target: 5'- aGCAUCAUCuGCAgugguuuuucgcaaGGGUGUuUGCCCGg -3' miRNA: 3'- -CGUAGUGGcCGUa-------------CCUACGcAUGGGC- -5' |
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| 27363 | 3' | -55.1 | NC_005857.1 | + | 19551 | 0.68 | 0.583819 |
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Target: 5'- cGCGUUGCCcuGgGUGGAcaGCGUGCCCc -3' miRNA: 3'- -CGUAGUGGc-CgUACCUa-CGCAUGGGc -5' |
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| 27363 | 3' | -55.1 | NC_005857.1 | + | 23177 | 0.68 | 0.583819 |
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Target: 5'- cGCGUUGCCcuGgGUGGAcaGCGUGCCCc -3' miRNA: 3'- -CGUAGUGGc-CgUACCUa-CGCAUGGGc -5' |
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| 27363 | 3' | -55.1 | NC_005857.1 | + | 17075 | 0.68 | 0.583819 |
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Target: 5'- -aAUCACCGGCGUGGcccaCG-AUCCGg -3' miRNA: 3'- cgUAGUGGCCGUACCuac-GCaUGGGC- -5' |
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| 27363 | 3' | -55.1 | NC_005857.1 | + | 1978 | 0.69 | 0.5512 |
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Target: 5'- -uGUCACCGGCAaugaaggacUGGAaGCGgcucUugCCGg -3' miRNA: 3'- cgUAGUGGCCGU---------ACCUaCGC----AugGGC- -5' |
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| 27363 | 3' | -55.1 | NC_005857.1 | + | 35456 | 0.69 | 0.508639 |
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Target: 5'- cGCGUCugUGGcCAUaGGUGCG-ACCUGg -3' miRNA: 3'- -CGUAGugGCC-GUAcCUACGCaUGGGC- -5' |
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| 27363 | 3' | -55.1 | NC_005857.1 | + | 37451 | 0.7 | 0.487879 |
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Target: 5'- gGCG-CAUCGGUAUGGcugGC-UACCCGg -3' miRNA: 3'- -CGUaGUGGCCGUACCua-CGcAUGGGC- -5' |
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| 27363 | 3' | -55.1 | NC_005857.1 | + | 8082 | 0.72 | 0.390881 |
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Target: 5'- cGCGUCGCCGGguUucccccauuuGGuuaGCGUGCCUGc -3' miRNA: 3'- -CGUAGUGGCCguA----------CCua-CGCAUGGGC- -5' |
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| 27363 | 3' | -55.1 | NC_005857.1 | + | 7163 | 1.1 | 0.000807 |
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Target: 5'- cGCAUCACCGGCAUGGAUGCGUACCCGc -3' miRNA: 3'- -CGUAGUGGCCGUACCUACGCAUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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