Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27370 | 5' | -57.7 | NC_005857.1 | + | 34525 | 0.67 | 0.42132 |
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Target: 5'- -cGGUGGCGGUAccGGAgGCCUG-GGCg- -3' miRNA: 3'- aaUUACCGUCGU--CCU-CGGACgCCGac -5' |
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| 27370 | 5' | -57.7 | NC_005857.1 | + | 2715 | 0.67 | 0.411719 |
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Target: 5'- uUUGAgcaGCAGCAGGGGUugCUGgGGCUu -3' miRNA: 3'- -AAUUac-CGUCGUCCUCG--GACgCCGAc -5' |
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| 27370 | 5' | -57.7 | NC_005857.1 | + | 20655 | 0.7 | 0.287994 |
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Target: 5'- -gGGUGGCAGacgacagucgguauuCAGGGGCagaaUGCGGCg- -3' miRNA: 3'- aaUUACCGUC---------------GUCCUCGg---ACGCCGac -5' |
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| 27370 | 5' | -57.7 | NC_005857.1 | + | 24281 | 0.7 | 0.287994 |
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Target: 5'- -gGGUGGCAGacgauagucgguauuCAGGGGCagaaUGCGGCg- -3' miRNA: 3'- aaUUACCGUC---------------GUCCUCGg---ACGCCGac -5' |
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| 27370 | 5' | -57.7 | NC_005857.1 | + | 24128 | 0.97 | 0.002528 |
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Target: 5'- uUUAAcGGCAGCAGGAGCCUGCGGCUGc -3' miRNA: 3'- -AAUUaCCGUCGUCCUCGGACGCCGAC- -5' |
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| 27370 | 5' | -57.7 | NC_005857.1 | + | 20502 | 1.05 | 0.00068 |
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Target: 5'- uUUAAUGGCAGCAGGAGCCUGCGGCUGc -3' miRNA: 3'- -AAUUACCGUCGUCCUCGGACGCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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