Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27372 | 5' | -51.5 | NC_005857.1 | + | 21297 | 0.72 | 0.515503 |
Target: 5'- cCCUguUGC-GUCACCGuauucuguaacgucuGCAGCGCCGUUGa -3' miRNA: 3'- -GGA--AUGaCGGUGGU---------------UGUCGCGGUAAU- -5' |
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27372 | 5' | -51.5 | NC_005857.1 | + | 23150 | 0.72 | 0.504558 |
Target: 5'- cCCcUGCUGCguCACCGuguucugcaggcuuuGCAGCGCCGUg- -3' miRNA: 3'- -GGaAUGACG--GUGGU---------------UGUCGCGGUAau -5' |
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27372 | 5' | -51.5 | NC_005857.1 | + | 42818 | 0.78 | 0.231046 |
Target: 5'- uUCUU-CUGCCACaCGAUAGCGCCAc-- -3' miRNA: 3'- -GGAAuGACGGUG-GUUGUCGCGGUaau -5' |
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27372 | 5' | -51.5 | NC_005857.1 | + | 21120 | 1 | 0.007605 |
Target: 5'- aUCUUACUGCCGCCAACAGCGCCAUc- -3' miRNA: 3'- -GGAAUGACGGUGGUUGUCGCGGUAau -5' |
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27372 | 5' | -51.5 | NC_005857.1 | + | 22972 | 1.09 | 0.001751 |
Target: 5'- aCCUUACUGCCACCAACAGCGCCAUUAc -3' miRNA: 3'- -GGAAUGACGGUGGUUGUCGCGGUAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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