Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27376 | 5' | -53.8 | NC_005857.1 | + | 25998 | 0.66 | 0.793131 |
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Target: 5'- uCC-GUGGgUUUUCCAuc-UCCAGCCg -3' miRNA: 3'- -GGaCACCgGAAAGGUcauAGGUCGGg -5' |
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| 27376 | 5' | -53.8 | NC_005857.1 | + | 6702 | 0.66 | 0.752335 |
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Target: 5'- ---cUGGCCa-UCCAGUAUCUGGUCa -3' miRNA: 3'- ggacACCGGaaAGGUCAUAGGUCGGg -5' |
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| 27376 | 5' | -53.8 | NC_005857.1 | + | 6238 | 0.67 | 0.741792 |
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Target: 5'- aUCUGUGGCgUcaCCGGUAUuacugCUGGCCg -3' miRNA: 3'- -GGACACCGgAaaGGUCAUA-----GGUCGGg -5' |
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| 27376 | 5' | -53.8 | NC_005857.1 | + | 7610 | 0.67 | 0.709528 |
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Target: 5'- -----uGCCgUUCCAGcucAUCCAGCCCu -3' miRNA: 3'- ggacacCGGaAAGGUCa--UAGGUCGGG- -5' |
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| 27376 | 5' | -53.8 | NC_005857.1 | + | 19227 | 0.68 | 0.654348 |
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Target: 5'- aCUGgcaucaGCCUUUuuccCCAGgcugguuUCCAGCCCg -3' miRNA: 3'- gGACac----CGGAAA----GGUCau-----AGGUCGGG- -5' |
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| 27376 | 5' | -53.8 | NC_005857.1 | + | 37366 | 0.7 | 0.565467 |
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Target: 5'- ----gGGUCUUUCCGGguagCCAGCCa -3' miRNA: 3'- ggacaCCGGAAAGGUCaua-GGUCGGg -5' |
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| 27376 | 5' | -53.8 | NC_005857.1 | + | 51386 | 0.7 | 0.562174 |
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Target: 5'- aUUGUGGCCagUCCGGgcauaacucuaaugCCuGCCCa -3' miRNA: 3'- gGACACCGGaaAGGUCaua-----------GGuCGGG- -5' |
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| 27376 | 5' | -53.8 | NC_005857.1 | + | 31294 | 0.7 | 0.522061 |
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Target: 5'- aCCUGUGGCCUUUUUgucacuugaAGUAggcacUCCAGgCa -3' miRNA: 3'- -GGACACCGGAAAGG---------UCAU-----AGGUCgGg -5' |
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| 27376 | 5' | -53.8 | NC_005857.1 | + | 31248 | 1.14 | 0.000611 |
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Target: 5'- gCCUGUGGCCUUUCCAGUAUCCAGCCCu -3' miRNA: 3'- -GGACACCGGAAAGGUCAUAGGUCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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