Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27377 | 5' | -48.1 | NC_005857.1 | + | 47342 | 0.66 | 0.967717 |
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Target: 5'- uGGCgauGCUguuUUGCCGCCu--GCGUuaucuGGCg -3' miRNA: 3'- -CCGau-UGA---AAUGGCGGuuuCGCG-----UUG- -5' |
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| 27377 | 5' | -48.1 | NC_005857.1 | + | 51480 | 0.68 | 0.929966 |
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Target: 5'- aGGC-AGCU--GCUGCCAGGGgGUAGg -3' miRNA: 3'- -CCGaUUGAaaUGGCGGUUUCgCGUUg -5' |
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| 27377 | 5' | -48.1 | NC_005857.1 | + | 8627 | 0.72 | 0.731896 |
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Target: 5'- uGGCUGAa---ACCGCaaaagcuGGCGCAGCg -3' miRNA: 3'- -CCGAUUgaaaUGGCGguu----UCGCGUUG- -5' |
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| 27377 | 5' | -48.1 | NC_005857.1 | + | 18481 | 0.72 | 0.720564 |
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Target: 5'- cGGCUGGCUgagACCGgCAAAccaUGCGACa -3' miRNA: 3'- -CCGAUUGAaa-UGGCgGUUUc--GCGUUG- -5' |
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| 27377 | 5' | -48.1 | NC_005857.1 | + | 8880 | 0.75 | 0.603966 |
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Target: 5'- uGGCUGGCcg-ACCGCUggGGCG-AGCc -3' miRNA: 3'- -CCGAUUGaaaUGGCGGuuUCGCgUUG- -5' |
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| 27377 | 5' | -48.1 | NC_005857.1 | + | 42516 | 0.79 | 0.396333 |
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Target: 5'- uGGCUGACUggcuacACCGCCuGGGUGUGGCu -3' miRNA: 3'- -CCGAUUGAaa----UGGCGGuUUCGCGUUG- -5' |
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| 27377 | 5' | -48.1 | NC_005857.1 | + | 32691 | 1.14 | 0.002136 |
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Target: 5'- gGGCUAACUUUACCGCCAAAGCGCAACg -3' miRNA: 3'- -CCGAUUGAAAUGGCGGUUUCGCGUUG- -5' |
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| 27377 | 5' | -48.1 | NC_005857.1 | + | 4980 | 0.66 | 0.975445 |
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Target: 5'- aGGCaUGACUccauggugaugucgaUUGCCGCU---GCGCAGg -3' miRNA: 3'- -CCG-AUUGA---------------AAUGGCGGuuuCGCGUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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