miRNA: 3'- aAAGUUauaGGGUAGcCUGUUcGACC- -5'
miRNA: 3'- -AAAgUUAUAGGGuaGCCUGuuCGACC- -5'
miRNA: 3'- -AAAGUUAUAGGgUag-CC---UGUUCGACC- -5'
miRNA: 3'- aaaGUUAUAGG-GUAGCCuGUUCGacc -5'
miRNA: 3'- -AAAGUUAUAGGGUAGCCUGUUCGACC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27378 | 5' | -47.9 | NC_005857.1 | + | 48440 | 0.67 | 0.959464 |
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Target: 5'- gUUCAAg--CCCGUCaGGCAGaCUGGc -3' miRNA: 3'- aAAGUUauaGGGUAGcCUGUUcGACC- -5' |
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| 27378 | 5' | -47.9 | NC_005857.1 | + | 41363 | 0.7 | 0.871097 |
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Target: 5'- aUUUgGAUGUUCUggCGGAUucGCUGGa -3' miRNA: 3'- -AAAgUUAUAGGGuaGCCUGuuCGACC- -5' |
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| 27378 | 5' | -47.9 | NC_005857.1 | + | 9253 | 0.71 | 0.81567 |
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Target: 5'- cUUUCGAUAUCCgGgauGGccaGCAGGCUGGc -3' miRNA: 3'- -AAAGUUAUAGGgUag-CC---UGUUCGACC- -5' |
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| 27378 | 5' | -47.9 | NC_005857.1 | + | 10408 | 0.71 | 0.805594 |
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Target: 5'- --gCAAUAUCCgCAUCGG-CAAGCg-- -3' miRNA: 3'- aaaGUUAUAGG-GUAGCCuGUUCGacc -5' |
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| 27378 | 5' | -47.9 | NC_005857.1 | + | 35277 | 1.1 | 0.004004 |
|
Target: 5'- uUUUCAAUAUCCCAUCGGACAAGCUGGg -3' miRNA: 3'- -AAAGUUAUAGGGUAGCCUGUUCGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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