Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27379 | 5' | -51.5 | NC_005857.1 | + | 34445 | 0.66 | 0.896783 |
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Target: 5'- aGCAUcgcGAgaaCGUUCGCguCGUUGUACa -3' miRNA: 3'- -CGUA---CUac-GCGAGCGguGCAACAUGg -5' |
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| 27379 | 5' | -51.5 | NC_005857.1 | + | 41033 | 0.66 | 0.87392 |
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Target: 5'- aGCA----GCuGCUCGCCACGgcGUuucuACCg -3' miRNA: 3'- -CGUacuaCG-CGAGCGGUGCaaCA----UGG- -5' |
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| 27379 | 5' | -51.5 | NC_005857.1 | + | 13541 | 0.66 | 0.864932 |
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Target: 5'- gGCAgcucguUGUGCUCGCCAUGacguaauUUGcagGCCg -3' miRNA: 3'- -CGUacu---ACGCGAGCGGUGC-------AACa--UGG- -5' |
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| 27379 | 5' | -51.5 | NC_005857.1 | + | 25515 | 0.68 | 0.780865 |
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Target: 5'- cGCAuuUGcgGCGCUCgaaaccgugucaGUCACGUUGguggcauUGCCg -3' miRNA: 3'- -CGU--ACuaCGCGAG------------CGGUGCAAC-------AUGG- -5' |
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| 27379 | 5' | -51.5 | NC_005857.1 | + | 42905 | 0.71 | 0.617169 |
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Target: 5'- aGCAUGcgGaaaGCUCGCCGCcga-UGCCa -3' miRNA: 3'- -CGUACuaCg--CGAGCGGUGcaacAUGG- -5' |
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| 27379 | 5' | -51.5 | NC_005857.1 | + | 11692 | 0.73 | 0.485174 |
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Target: 5'- gGCA-GAUGCGCaguaucagacuUCGCUcgaGUUGUACCg -3' miRNA: 3'- -CGUaCUACGCG-----------AGCGGug-CAACAUGG- -5' |
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| 27379 | 5' | -51.5 | NC_005857.1 | + | 36041 | 1.16 | 0.000815 |
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Target: 5'- aGCAUGAUGCGCUCGCCACGUUGUACCg -3' miRNA: 3'- -CGUACUACGCGAGCGGUGCAACAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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