Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2738 | 3' | -55.7 | NC_001491.2 | + | 139859 | 0.66 | 0.915308 |
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Target: 5'- cUCGCGCcCGCaGAuguacGAGCGCgcgugUGCGCGGu -3' miRNA: 3'- -AGCGUGcGCG-CUu----CUCGUG-----AUGCGCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 82291 | 0.66 | 0.915308 |
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Target: 5'- -gGUgACGC-UGGAGAGCAUcACGCGAc -3' miRNA: 3'- agCG-UGCGcGCUUCUCGUGaUGCGCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 84641 | 0.66 | 0.915308 |
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Target: 5'- -aGCACGCGUcAGGAacgauuauguGCACcagcGCGCGAg -3' miRNA: 3'- agCGUGCGCGcUUCU----------CGUGa---UGCGCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 148964 | 0.66 | 0.921008 |
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Target: 5'- -aGCGCG-GCGGAGGGC-CUGgGCc- -3' miRNA: 3'- agCGUGCgCGCUUCUCGuGAUgCGcu -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 9452 | 0.66 | 0.926468 |
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Target: 5'- -aGCGCGCuCGAGGGGUucauCGCGGc -3' miRNA: 3'- agCGUGCGcGCUUCUCGugauGCGCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 85076 | 0.66 | 0.926468 |
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Target: 5'- aCGcCACGaauuguCGacaGAGGAGUGCUACGCGc -3' miRNA: 3'- aGC-GUGC------GCg--CUUCUCGUGAUGCGCu -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 60081 | 0.66 | 0.926468 |
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Target: 5'- -aGCGCGUGCGGuaGGUGCcGCGCa- -3' miRNA: 3'- agCGUGCGCGCUucUCGUGaUGCGcu -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 52379 | 0.66 | 0.926468 |
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Target: 5'- cUUGUACGCGCGAcaucugguAGAucuuuuGUugUACGaCGAg -3' miRNA: 3'- -AGCGUGCGCGCU--------UCU------CGugAUGC-GCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 33161 | 0.66 | 0.926468 |
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Target: 5'- cUCGCGCGCcccaaGcAGAGCACgucCGUGGa -3' miRNA: 3'- -AGCGUGCGcg---CuUCUCGUGau-GCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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