Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
2738 | 3' | -55.7 | NC_001491.2 | + | 123068 | 0.68 | 0.811274 |
Target: 5'- gCGCACGacCGCGGcaaagucccccacGGAGCGCgGCaGCGAg -3' miRNA: 3'- aGCGUGC--GCGCU-------------UCUCGUGaUG-CGCU- -5' |
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2738 | 3' | -55.7 | NC_001491.2 | + | 20922 | 0.69 | 0.794349 |
Target: 5'- aCGCAacaucaGCGgGGugGGGGCAgUAUGCGAa -3' miRNA: 3'- aGCGUg-----CGCgCU--UCUCGUgAUGCGCU- -5' |
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2738 | 3' | -55.7 | NC_001491.2 | + | 82607 | 0.69 | 0.785226 |
Target: 5'- -aGC-CGCGCGAuGAguucgcGCGCUuGCGCGAg -3' miRNA: 3'- agCGuGCGCGCUuCU------CGUGA-UGCGCU- -5' |
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2738 | 3' | -55.7 | NC_001491.2 | + | 54685 | 0.7 | 0.718014 |
Target: 5'- cUCGUGCGCGCaauGGuGgACUGCGCGc -3' miRNA: 3'- -AGCGUGCGCGcu-UCuCgUGAUGCGCu -5' |
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2738 | 3' | -55.7 | NC_001491.2 | + | 36408 | 0.71 | 0.687921 |
Target: 5'- uUCGCGCGuCGCG-AGAGaauCAUUAcCGCGAa -3' miRNA: 3'- -AGCGUGC-GCGCuUCUC---GUGAU-GCGCU- -5' |
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2738 | 3' | -55.7 | NC_001491.2 | + | 147760 | 0.71 | 0.687921 |
Target: 5'- -aGCGCGCGCcgucccgggccGggGGGCcGCUGCaGCGGg -3' miRNA: 3'- agCGUGCGCG-----------CuuCUCG-UGAUG-CGCU- -5' |
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2738 | 3' | -55.7 | NC_001491.2 | + | 123308 | 0.72 | 0.607282 |
Target: 5'- -aGUACGCGCaGAAGAGCAUgcggccgcugacucgGCGUGGg -3' miRNA: 3'- agCGUGCGCG-CUUCUCGUGa--------------UGCGCU- -5' |
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2738 | 3' | -55.7 | NC_001491.2 | + | 14255 | 0.75 | 0.446867 |
Target: 5'- uUCGCGCGUGCGAAGcucugauagguaaaAGCGCUGUGcCGAu -3' miRNA: 3'- -AGCGUGCGCGCUUC--------------UCGUGAUGC-GCU- -5' |
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2738 | 3' | -55.7 | NC_001491.2 | + | 68737 | 0.77 | 0.371752 |
Target: 5'- cCGCGCGUgcaGCGGAGAGCGCcgACGCc- -3' miRNA: 3'- aGCGUGCG---CGCUUCUCGUGa-UGCGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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