Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2738 | 3' | -55.7 | NC_001491.2 | + | 54685 | 0.7 | 0.718014 |
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Target: 5'- cUCGUGCGCGCaauGGuGgACUGCGCGc -3' miRNA: 3'- -AGCGUGCGCGcu-UCuCgUGAUGCGCu -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 52379 | 0.66 | 0.926468 |
|
Target: 5'- cUUGUACGCGCGAcaucugguAGAucuuuuGUugUACGaCGAg -3' miRNA: 3'- -AGCGUGCGCGCU--------UCU------CGugAUGC-GCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 38639 | 0.67 | 0.876199 |
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Target: 5'- -gGUGCGCGCuuuccuGAAGAGCGaccCUAUGCGc -3' miRNA: 3'- agCGUGCGCG------CUUCUCGU---GAUGCGCu -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 36408 | 0.71 | 0.687921 |
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Target: 5'- uUCGCGCGuCGCG-AGAGaauCAUUAcCGCGAa -3' miRNA: 3'- -AGCGUGC-GCGCuUCUC---GUGAU-GCGCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 33161 | 0.66 | 0.926468 |
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Target: 5'- cUCGCGCGCcccaaGcAGAGCACgucCGUGGa -3' miRNA: 3'- -AGCGUGCGcg---CuUCUCGUGau-GCGCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 20922 | 0.69 | 0.794349 |
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Target: 5'- aCGCAacaucaGCGgGGugGGGGCAgUAUGCGAa -3' miRNA: 3'- aGCGUg-----CGCgCU--UCUCGUgAUGCGCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 15772 | 0.66 | 0.908764 |
|
Target: 5'- gUGCAaagucCGCGCGAGGucugggugcuuguGGCugUGCGgGGa -3' miRNA: 3'- aGCGU-----GCGCGCUUC-------------UCGugAUGCgCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 14255 | 0.75 | 0.446867 |
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Target: 5'- uUCGCGCGUGCGAAGcucugauagguaaaAGCGCUGUGcCGAu -3' miRNA: 3'- -AGCGUGCGCGCUUC--------------UCGUGAUGC-GCU- -5' |
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| 2738 | 3' | -55.7 | NC_001491.2 | + | 9452 | 0.66 | 0.926468 |
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Target: 5'- -aGCGCGCuCGAGGGGUucauCGCGGc -3' miRNA: 3'- agCGUGCGcGCUUCUCGugauGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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