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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2738 | 5' | -49.2 | NC_001491.2 | + | 6238 | 0.66 | 0.997196 |
Target: 5'- -cUUGUGauaGGCGCUGUgucgauucuacUGCACUACg- -3' miRNA: 3'- auAACGCa--UCGCGAUA-----------ACGUGAUGga -5' |
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2738 | 5' | -49.2 | NC_001491.2 | + | 36183 | 0.67 | 0.993662 |
Target: 5'- aGUUGCuuuGCGCUccUGUugUACCUc -3' miRNA: 3'- aUAACGcauCGCGAuaACGugAUGGA- -5' |
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2738 | 5' | -49.2 | NC_001491.2 | + | 26881 | 0.68 | 0.987204 |
Target: 5'- ---cGCGUGGaGCUAUgcucGCACUugCg -3' miRNA: 3'- auaaCGCAUCgCGAUAa---CGUGAugGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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