miRNA: 3'- aCU--UUAgUGCUACGGACaua-------GACUUg -5'
miRNA: 3'- -ACUUUAGUGCU-----------ACGGACAu--AGACUug -5'
miRNA: 3'- -ACuuUAGUgCu--ACGGaCAUAGACUUG- -5'
miRNA: 3'- aCUu--UAGUGCU-ACGGACaUAGACUUG- -5'
miRNA: 3'- -ACUUUAGUGCUACGGACAUAGACUUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27381 | 3' | -45.6 | NC_005857.1 | + | 15762 | 0.66 | 0.994056 |
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Target: 5'- aGAccGAUgGCGGUGCCUGgcagaccgugCUGGAa -3' miRNA: 3'- aCU--UUAgUGCUACGGACaua-------GACUUg -5' |
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| 27381 | 3' | -45.6 | NC_005857.1 | + | 35919 | 0.68 | 0.975904 |
|
Target: 5'- gUGAAAUCAUGAcuucuuucucuUGCUUGUuuuUCUGGu- -3' miRNA: 3'- -ACUUUAGUGCU-----------ACGGACAu--AGACUug -5' |
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| 27381 | 3' | -45.6 | NC_005857.1 | + | 43404 | 0.71 | 0.912878 |
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Target: 5'- aUGugGUCAgGuauUGCCgGUAUCUGAAUg -3' miRNA: 3'- -ACuuUAGUgCu--ACGGaCAUAGACUUG- -5' |
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| 27381 | 3' | -45.6 | NC_005857.1 | + | 4667 | 0.75 | 0.765397 |
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Target: 5'- aGGuccAUCGCGGcUGCCUGcAUCUGAAUa -3' miRNA: 3'- aCUu--UAGUGCU-ACGGACaUAGACUUG- -5' |
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| 27381 | 3' | -45.6 | NC_005857.1 | + | 39985 | 1.13 | 0.005023 |
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Target: 5'- aUGAAAUCACGAUGCCUGUAUCUGAACa -3' miRNA: 3'- -ACUUUAGUGCUACGGACAUAGACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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