Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27381 | 5' | -53.6 | NC_005857.1 | + | 38157 | 0.66 | 0.734111 |
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Target: 5'- -cGCGCGGCGGGGAAGaGCGu----- -3' miRNA: 3'- uuUGCGUCGCCUCUUCgCGUcacuuu -5' |
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| 27381 | 5' | -53.6 | NC_005857.1 | + | 332 | 0.66 | 0.734111 |
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Target: 5'- ---aGCAGcCGGAGGccagaaacuuGGCGCAgccGUGAAAg -3' miRNA: 3'- uuugCGUC-GCCUCU----------UCGCGU---CACUUU- -5' |
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| 27381 | 5' | -53.6 | NC_005857.1 | + | 20504 | 0.66 | 0.734111 |
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Target: 5'- uAAugGCAGCaGGAGccugcggcuGCGCcGUGAAGc -3' miRNA: 3'- -UUugCGUCG-CCUCuu-------CGCGuCACUUU- -5' |
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| 27381 | 5' | -53.6 | NC_005857.1 | + | 6590 | 0.66 | 0.723011 |
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Target: 5'- -cGCGCAGCGGAGcaucGAGCGUg------ -3' miRNA: 3'- uuUGCGUCGCCUC----UUCGCGucacuuu -5' |
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| 27381 | 5' | -53.6 | NC_005857.1 | + | 17254 | 0.66 | 0.721895 |
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Target: 5'- gGAAUGCGGUGaaaGAugcgauuucguauGAAGCGCAGUGGc- -3' miRNA: 3'- -UUUGCGUCGC---CU-------------CUUCGCGUCACUuu -5' |
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| 27381 | 5' | -53.6 | NC_005857.1 | + | 20027 | 0.7 | 0.464243 |
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Target: 5'- cAGCGCGGCGGcAGuGAGCGCccugacaucccgGGUGGAu -3' miRNA: 3'- uUUGCGUCGCC-UC-UUCGCG------------UCACUUu -5' |
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| 27381 | 5' | -53.6 | NC_005857.1 | + | 23654 | 0.7 | 0.464243 |
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Target: 5'- cAGCGCGGCGGcAGuGAGCGCccugacaucccgGGUGGAu -3' miRNA: 3'- uUUGCGUCGCC-UC-UUCGCG------------UCACUUu -5' |
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| 27381 | 5' | -53.6 | NC_005857.1 | + | 40025 | 1.03 | 0.002274 |
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Target: 5'- uAAACGCAGCGGAGAAGCGCAGUGAAAu -3' miRNA: 3'- -UUUGCGUCGCCUCUUCGCGUCACUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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