miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27384 3' -53.7 NC_005857.1 + 15908 0.67 0.679075
Target:  5'- uGCGGCAGGUCgaUGCgGuGGCUGCGc-- -3'
miRNA:   3'- -UGUUGUCCAGa-ACGgC-UCGGCGUaug -5'
27384 3' -53.7 NC_005857.1 + 18333 0.68 0.6452
Target:  5'- aGCAGCuGGUC-UGCCGccugcGCCGCuuucGCg -3'
miRNA:   3'- -UGUUGuCCAGaACGGCu----CGGCGua--UG- -5'
27384 3' -53.7 NC_005857.1 + 19612 0.75 0.254623
Target:  5'- gGCAACGGGgugauggcauucacgCUgaaaGCCgGAGCCGCAUACc -3'
miRNA:   3'- -UGUUGUCCa--------------GAa---CGG-CUCGGCGUAUG- -5'
27384 3' -53.7 NC_005857.1 + 23238 0.75 0.254623
Target:  5'- gGCAACGGGgugauggcauucacgCUgaaaGCCgGAGCCGCAUACc -3'
miRNA:   3'- -UGUUGUCCa--------------GAa---CGG-CUCGGCGUAUG- -5'
27384 3' -53.7 NC_005857.1 + 46268 1.1 0.000921
Target:  5'- aACAACAGGUCUUGCCGAGCCGCAUACg -3'
miRNA:   3'- -UGUUGUCCAGAACGGCUCGGCGUAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.