Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27386 | 3' | -54.3 | NC_005857.1 | + | 8196 | 0.66 | 0.761738 |
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Target: 5'- uGCGAGCUuuacggcguuacuuaAUGCgGCAgagGCUCGCGAg- -3' miRNA: 3'- gCGCUUGA---------------UGCG-CGUa--CGGGCGUUac -5' |
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| 27386 | 3' | -54.3 | NC_005857.1 | + | 3952 | 0.66 | 0.757573 |
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Target: 5'- uCGCGGACaaauCaCGCAgGCCUGCAcgGa -3' miRNA: 3'- -GCGCUUGau--GcGCGUaCGGGCGUuaC- -5' |
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| 27386 | 3' | -54.3 | NC_005857.1 | + | 47872 | 0.68 | 0.626196 |
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Target: 5'- uGCGGGCaUGCGCGUA-GUUCGCGGa- -3' miRNA: 3'- gCGCUUG-AUGCGCGUaCGGGCGUUac -5' |
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| 27386 | 3' | -54.3 | NC_005857.1 | + | 47719 | 0.68 | 0.623961 |
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Target: 5'- gGCGcAGCUGCGUGCcguacuucccCCUGCAGUGa -3' miRNA: 3'- gCGC-UUGAUGCGCGuac-------GGGCGUUAC- -5' |
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| 27386 | 3' | -54.3 | NC_005857.1 | + | 34503 | 0.69 | 0.55957 |
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Target: 5'- gGCGGAgaACGCGC-UGCCaGCAAc- -3' miRNA: 3'- gCGCUUgaUGCGCGuACGGgCGUUac -5' |
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| 27386 | 3' | -54.3 | NC_005857.1 | + | 47799 | 1.08 | 0.001305 |
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Target: 5'- cCGCGAACUACGCGCAUGCCCGCAAUGc -3' miRNA: 3'- -GCGCUUGAUGCGCGUACGGGCGUUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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