Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27387 | 3' | -53.7 | NC_005857.1 | + | 12470 | 0.66 | 0.7866 |
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Target: 5'- -uUCCAgaUAgcaCCGcCCGGUCGCAUAu- -3' miRNA: 3'- cuAGGUg-AUa--GGC-GGCCAGCGUAUcg -5' |
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| 27387 | 3' | -53.7 | NC_005857.1 | + | 15440 | 0.67 | 0.745301 |
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Target: 5'- cAUCCgGCUGcUCCGUCGGcgugCGC-UGGCu -3' miRNA: 3'- cUAGG-UGAU-AGGCGGCCa---GCGuAUCG- -5' |
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| 27387 | 3' | -53.7 | NC_005857.1 | + | 31162 | 0.68 | 0.646611 |
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Target: 5'- uGGUCCGgUG-CCGCCGGuaaUCGgGUAGa -3' miRNA: 3'- -CUAGGUgAUaGGCGGCC---AGCgUAUCg -5' |
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| 27387 | 3' | -53.7 | NC_005857.1 | + | 6256 | 0.69 | 0.590759 |
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Target: 5'- --aCCACUA-CCGUCGG-CGCcaucuGUGGCg -3' miRNA: 3'- cuaGGUGAUaGGCGGCCaGCG-----UAUCG- -5' |
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| 27387 | 3' | -53.7 | NC_005857.1 | + | 48609 | 0.7 | 0.568604 |
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Target: 5'- -uUCCGCUAUgCGaCCGG-CGgAUAGUg -3' miRNA: 3'- cuAGGUGAUAgGC-GGCCaGCgUAUCG- -5' |
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| 27387 | 3' | -53.7 | NC_005857.1 | + | 35196 | 0.75 | 0.274996 |
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Target: 5'- uGAUCCACUuuaCCuacgcuuaccgcgugGCCGGUCGC-UGGCa -3' miRNA: 3'- -CUAGGUGAua-GG---------------CGGCCAGCGuAUCG- -5' |
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| 27387 | 3' | -53.7 | NC_005857.1 | + | 48528 | 1.13 | 0.000703 |
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Target: 5'- cGAUCCACUAUCCGCCGGUCGCAUAGCg -3' miRNA: 3'- -CUAGGUGAUAGGCGGCCAGCGUAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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