Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27387 | 5' | -57.8 | NC_005857.1 | + | 48408 | 0.67 | 0.515432 |
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Target: 5'- aGGACCGUGUGGCCGugcCGGgucaaUCGguCGGc -3' miRNA: 3'- -UUUGGCACGCUGGU---GCC-----AGCguGCCc -5' |
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| 27387 | 5' | -57.8 | NC_005857.1 | + | 12194 | 0.68 | 0.455287 |
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Target: 5'- uGACgCGUGcCGGugACGGcUCGCugGGGg -3' miRNA: 3'- uUUG-GCAC-GCUggUGCC-AGCGugCCC- -5' |
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| 27387 | 5' | -57.8 | NC_005857.1 | + | 17717 | 0.69 | 0.417363 |
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Target: 5'- --uCCGUGgaCGGCCugGGU-GCAgGGGa -3' miRNA: 3'- uuuGGCAC--GCUGGugCCAgCGUgCCC- -5' |
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| 27387 | 5' | -57.8 | NC_005857.1 | + | 37941 | 0.71 | 0.308448 |
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Target: 5'- gAAAUCaUGCGACCGUGGUCGCACa-- -3' miRNA: 3'- -UUUGGcACGCUGGUGCCAGCGUGccc -5' |
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| 27387 | 5' | -57.8 | NC_005857.1 | + | 40376 | 0.74 | 0.211366 |
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Target: 5'- --uCUGUGCGACCGUGGUCGCACc-- -3' miRNA: 3'- uuuGGCACGCUGGUGCCAGCGUGccc -5' |
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| 27387 | 5' | -57.8 | NC_005857.1 | + | 45410 | 0.77 | 0.123666 |
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Target: 5'- --uCUGUGCGACCGUGGUCGCACGc- -3' miRNA: 3'- uuuGGCACGCUGGUGCCAGCGUGCcc -5' |
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| 27387 | 5' | -57.8 | NC_005857.1 | + | 40304 | 0.8 | 0.079217 |
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Target: 5'- ----gGUGCGACCACGGUCGCACa-- -3' miRNA: 3'- uuuggCACGCUGGUGCCAGCGUGccc -5' |
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| 27387 | 5' | -57.8 | NC_005857.1 | + | 45333 | 0.86 | 0.026687 |
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Target: 5'- aAAAgCGUGCGACCACGGUCGCACa-- -3' miRNA: 3'- -UUUgGCACGCUGGUGCCAGCGUGccc -5' |
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| 27387 | 5' | -57.8 | NC_005857.1 | + | 48640 | 0.87 | 0.023086 |
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Target: 5'- --cCCGUGCGACCGUGGUCGCACGGu -3' miRNA: 3'- uuuGGCACGCUGGUGCCAGCGUGCCc -5' |
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| 27387 | 5' | -57.8 | NC_005857.1 | + | 37862 | 0.9 | 0.015366 |
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Target: 5'- cAGGCUGUGCGACCACGGUCGCAUGa- -3' miRNA: 3'- -UUUGGCACGCUGGUGCCAGCGUGCcc -5' |
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| 27387 | 5' | -57.8 | NC_005857.1 | + | 48564 | 1.07 | 0.000753 |
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Target: 5'- gAAACCGUGCGACCACGGUCGCACGGGu -3' miRNA: 3'- -UUUGGCACGCUGGUGCCAGCGUGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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