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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27389 | 3' | -51.7 | NC_005857.1 | + | 42516 | 0.66 | 0.844485 |
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Target: 5'- uGGCUGacuggcuacaccGCCugGGUGugGcuAUCGAGGa -3' miRNA: 3'- uUCGAC------------CGGugCUACugU--UAGCUUCa -5' |
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| 27389 | 3' | -51.7 | NC_005857.1 | + | 18918 | 0.66 | 0.835281 |
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Target: 5'- -cGCUGGCCACGcaGAUg--CGggGg -3' miRNA: 3'- uuCGACCGGUGCuaCUGuuaGCuuCa -5' |
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| 27389 | 3' | -51.7 | NC_005857.1 | + | 12395 | 0.67 | 0.772355 |
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Target: 5'- -uGCUGGCCAaacuccucgaauccCGAUGuacuauCGGUUGAAGUu -3' miRNA: 3'- uuCGACCGGU--------------GCUACu-----GUUAGCUUCA- -5' |
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| 27389 | 3' | -51.7 | NC_005857.1 | + | 36095 | 0.68 | 0.754161 |
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Target: 5'- -uGCUGGCCAC--UGACAGUucuacguucgcCGAGGa -3' miRNA: 3'- uuCGACCGGUGcuACUGUUA-----------GCUUCa -5' |
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| 27389 | 3' | -51.7 | NC_005857.1 | + | 48268 | 0.68 | 0.743269 |
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Target: 5'- -cGCUGGCC-CGAUGACcgggcgCGAGa- -3' miRNA: 3'- uuCGACCGGuGCUACUGuua---GCUUca -5' |
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| 27389 | 3' | -51.7 | NC_005857.1 | + | 7441 | 0.7 | 0.618167 |
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Target: 5'- cAGCUGGCCAUGA--ACGAUUGAc-- -3' miRNA: 3'- uUCGACCGGUGCUacUGUUAGCUuca -5' |
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| 27389 | 3' | -51.7 | NC_005857.1 | + | 30971 | 0.7 | 0.618167 |
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Target: 5'- -cGCUGGaUgACGAUGA-GAUCGAAGUg -3' miRNA: 3'- uuCGACC-GgUGCUACUgUUAGCUUCA- -5' |
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| 27389 | 3' | -51.7 | NC_005857.1 | + | 50112 | 1.06 | 0.002804 |
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Target: 5'- cAAGCUGGCCACGAUGACAAUCGAAGUg -3' miRNA: 3'- -UUCGACCGGUGCUACUGUUAGCUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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