Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27391 | 3' | -47.4 | NC_005859.1 | + | 51485 | 0.69 | 0.988043 |
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Target: 5'- uAGCacGUUGUUGCUUA--ACCUGCa -3' miRNA: 3'- cUCGccUAACAACGAAUaaUGGACGg -5' |
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| 27391 | 3' | -47.4 | NC_005859.1 | + | 50268 | 0.71 | 0.961992 |
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Target: 5'- cAGCGGGUUG-UGCUUcuggUGgUUGCCa -3' miRNA: 3'- cUCGCCUAACaACGAAua--AUgGACGG- -5' |
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| 27391 | 3' | -47.4 | NC_005859.1 | + | 4499 | 0.73 | 0.929021 |
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Target: 5'- aGGgGGAUUGUUcCgcAUUACCUGCUg -3' miRNA: 3'- cUCgCCUAACAAcGaaUAAUGGACGG- -5' |
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| 27391 | 3' | -47.4 | NC_005859.1 | + | 115918 | 0.73 | 0.929021 |
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Target: 5'- aGGgGGAUUGUUcCgcAUUACCUGCUg -3' miRNA: 3'- cUCgCCUAACAAcGaaUAAUGGACGG- -5' |
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| 27391 | 3' | -47.4 | NC_005859.1 | + | 4683 | 1.15 | 0.006179 |
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Target: 5'- cGAGCGGAUUGUUGCUUAUUACCUGCCa -3' miRNA: 3'- -CUCGCCUAACAACGAAUAAUGGACGG- -5' |
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| 27391 | 3' | -47.4 | NC_005859.1 | + | 116102 | 1.15 | 0.006179 |
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Target: 5'- cGAGCGGAUUGUUGCUUAUUACCUGCCa -3' miRNA: 3'- -CUCGCCUAACAACGAAUAAUGGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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