Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27391 | 5' | -50.9 | NC_005859.1 | + | 12318 | 0.66 | 0.984719 |
Target: 5'- gGCAauCGGGgccaauuacAAUGUGGAACAGUCCUa -3' miRNA: 3'- -UGUc-GUCCa--------UUACGCCUUGUUAGGGg -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 3677 | 0.67 | 0.975446 |
Target: 5'- cCAGUcaaAGGgaaauuaUAGUGgGGAAUAuUCCCCa -3' miRNA: 3'- uGUCG---UCC-------AUUACgCCUUGUuAGGGG- -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 115096 | 0.67 | 0.975446 |
Target: 5'- cCAGUcaaAGGgaaauuaUAGUGgGGAAUAuUCCCCa -3' miRNA: 3'- uGUCG---UCC-------AUUACgCCUUGUuAGGGG- -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 51616 | 0.68 | 0.951539 |
Target: 5'- gGCAGUuuuccGG-AAUGCGGGAaug-CCCCa -3' miRNA: 3'- -UGUCGu----CCaUUACGCCUUguuaGGGG- -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 10060 | 0.68 | 0.947102 |
Target: 5'- gGC-GCAGGauUGGUGCGGAGCA--CCUg -3' miRNA: 3'- -UGuCGUCC--AUUACGCCUUGUuaGGGg -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 121480 | 0.68 | 0.947102 |
Target: 5'- gGC-GCAGGauUGGUGCGGAGCA--CCUg -3' miRNA: 3'- -UGuCGUCC--AUUACGCCUUGUuaGGGg -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 57119 | 0.69 | 0.926732 |
Target: 5'- cGCAGCA-GUAcgGUuaagaauagGGAAUAAUUCCCa -3' miRNA: 3'- -UGUCGUcCAUuaCG---------CCUUGUUAGGGG- -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 3860 | 0.72 | 0.81132 |
Target: 5'- aGCcGCAGGcgaauaaauuuaauUAGUGgGGAAUAuUCCCCa -3' miRNA: 3'- -UGuCGUCC--------------AUUACgCCUUGUuAGGGG- -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 115279 | 0.72 | 0.81132 |
Target: 5'- aGCcGCAGGcgaauaaauuuaauUAGUGgGGAAUAuUCCCCa -3' miRNA: 3'- -UGuCGUCC--------------AUUACgCCUUGUuAGGGG- -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 112451 | 0.77 | 0.543458 |
Target: 5'- aAUAGCGGGUAAUGCuGAAUuaaucuAAUCCCUc -3' miRNA: 3'- -UGUCGUCCAUUACGcCUUG------UUAGGGG- -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 1031 | 0.77 | 0.543458 |
Target: 5'- aAUAGCGGGUAAUGCuGAAUuaaucuAAUCCCUc -3' miRNA: 3'- -UGUCGUCCAUUACGcCUUG------UUAGGGG- -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 35381 | 0.77 | 0.53298 |
Target: 5'- -aGGUAGGguuUGCGGGuuCGAUCCCCa -3' miRNA: 3'- ugUCGUCCauuACGCCUu-GUUAGGGG- -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 4648 | 1.11 | 0.004723 |
Target: 5'- uACAGCAGGUAAUGCGGAACAAUCCCCc -3' miRNA: 3'- -UGUCGUCCAUUACGCCUUGUUAGGGG- -5' |
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27391 | 5' | -50.9 | NC_005859.1 | + | 116067 | 1.11 | 0.004723 |
Target: 5'- uACAGCAGGUAAUGCGGAACAAUCCCCc -3' miRNA: 3'- -UGUCGUCCAUUACGCCUUGUUAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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