Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27393 | 3' | -54.9 | NC_005859.1 | + | 7486 | 0.66 | 0.915841 |
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Target: 5'- uUCGCCUUUUGGUUgcuaGgGGCguaCUGGUCa -3' miRNA: 3'- -AGUGGAAAGUCAGg---CaCCGa--GACCGG- -5' |
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| 27393 | 3' | -54.9 | NC_005859.1 | + | 118905 | 0.66 | 0.915841 |
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Target: 5'- uUCGCCUUUUGGUUgcuaGgGGCguaCUGGUCa -3' miRNA: 3'- -AGUGGAAAGUCAGg---CaCCGa--GACCGG- -5' |
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| 27393 | 3' | -54.9 | NC_005859.1 | + | 92224 | 0.74 | 0.497525 |
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Target: 5'- aCugCUUUCAGagaugCCGUGGaaCUGGCUc -3' miRNA: 3'- aGugGAAAGUCa----GGCACCgaGACCGG- -5' |
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| 27393 | 3' | -54.9 | NC_005859.1 | + | 83674 | 0.74 | 0.47226 |
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Target: 5'- gUCGCCUgucugguaaugagcuUUCGGUUgGUGGCgCUGGCg -3' miRNA: 3'- -AGUGGA---------------AAGUCAGgCACCGaGACCGg -5' |
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| 27393 | 3' | -54.9 | NC_005859.1 | + | 72804 | 0.75 | 0.449527 |
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Target: 5'- aUCACC-UUCGGuauUCUGUauGGUUCUGGCCc -3' miRNA: 3'- -AGUGGaAAGUC---AGGCA--CCGAGACCGG- -5' |
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| 27393 | 3' | -54.9 | NC_005859.1 | + | 79790 | 1.13 | 0.001477 |
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Target: 5'- gUCACCUUUCAGUCCGUGGCUCUGGCCa -3' miRNA: 3'- -AGUGGAAAGUCAGGCACCGAGACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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