Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27395 | 3' | -55.6 | NC_005859.1 | + | 17514 | 0.67 | 0.805008 |
Target: 5'- uGCCCguugaaaACACCGCCAGUAUcUA-GGUa -3' miRNA: 3'- gUGGG-------UGUGGCGGUCGUAaAUgCCGc -5' |
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27395 | 3' | -55.6 | NC_005859.1 | + | 64385 | 0.67 | 0.805008 |
Target: 5'- aGCCCACGCCGaCCuucgcgcAGCAUUcucugaUGCuGGCu -3' miRNA: 3'- gUGGGUGUGGC-GG-------UCGUAA------AUG-CCGc -5' |
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27395 | 3' | -55.6 | NC_005859.1 | + | 7335 | 0.68 | 0.7968 |
Target: 5'- uGCCCGCuuCCaGUuuugCAGCAUUUacaACGGCGg -3' miRNA: 3'- gUGGGUGu-GG-CG----GUCGUAAA---UGCCGC- -5' |
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27395 | 3' | -55.6 | NC_005859.1 | + | 118755 | 0.68 | 0.7968 |
Target: 5'- uGCCCGCuuCCaGUuuugCAGCAUUUacaACGGCGg -3' miRNA: 3'- gUGGGUGu-GG-CG----GUCGUAAA---UGCCGC- -5' |
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27395 | 3' | -55.6 | NC_005859.1 | + | 94218 | 0.73 | 0.493445 |
Target: 5'- cCACCaAUACCGCCAGCAguaAgGGCa -3' miRNA: 3'- -GUGGgUGUGGCGGUCGUaaaUgCCGc -5' |
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27395 | 3' | -55.6 | NC_005859.1 | + | 1996 | 1.03 | 0.006097 |
Target: 5'- aCACaCCACACCGCCAGCAUUUACGGCGg -3' miRNA: 3'- -GUG-GGUGUGGCGGUCGUAAAUGCCGC- -5' |
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27395 | 3' | -55.6 | NC_005859.1 | + | 113414 | 1.1 | 0.002078 |
Target: 5'- aCACCCACACCGCCAGCAUUUACGGCGg -3' miRNA: 3'- -GUGGGUGUGGCGGUCGUAAAUGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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