Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27398 | 3' | -46.2 | NC_005859.1 | + | 63980 | 0.69 | 0.995156 |
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Target: 5'- -aUGCUCGCUGUUGGccUGGUGGaGUAAc -3' miRNA: 3'- uaAUGGGCGAUAAUUu-AUCGCC-CAUU- -5' |
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| 27398 | 3' | -46.2 | NC_005859.1 | + | 4514 | 0.72 | 0.960888 |
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Target: 5'- cAUUACCUGCUGUaaaaguAGUGGCaGGUAAu -3' miRNA: 3'- -UAAUGGGCGAUAau----UUAUCGcCCAUU- -5' |
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| 27398 | 3' | -46.2 | NC_005859.1 | + | 115933 | 0.72 | 0.960888 |
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Target: 5'- cAUUACCUGCUGUaaaaguAGUGGCaGGUAAu -3' miRNA: 3'- -UAAUGGGCGAUAau----UUAUCGcCCAUU- -5' |
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| 27398 | 3' | -46.2 | NC_005859.1 | + | 1046 | 0.96 | 0.092129 |
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Target: 5'- uAUUACCCGCUAUUuAAUAGCGGGUAAu -3' miRNA: 3'- -UAAUGGGCGAUAAuUUAUCGCCCAUU- -5' |
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| 27398 | 3' | -46.2 | NC_005859.1 | + | 112466 | 0.96 | 0.092129 |
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Target: 5'- uAUUACCCGCUAUUuAAUAGCGGGUAAu -3' miRNA: 3'- -UAAUGGGCGAUAAuUUAUCGCCCAUU- -5' |
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| 27398 | 3' | -46.2 | NC_005859.1 | + | 898 | 1.05 | 0.029227 |
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Target: 5'- cAUUACCCGCUAUUAAAUAGCGGGUAAu -3' miRNA: 3'- -UAAUGGGCGAUAAUUUAUCGCCCAUU- -5' |
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| 27398 | 3' | -46.2 | NC_005859.1 | + | 112317 | 1.05 | 0.029227 |
|
Target: 5'- cAUUACCCGCUAUUAAAUAGCGGGUAAu -3' miRNA: 3'- -UAAUGGGCGAUAAUUUAUCGCCCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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