Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27398 | 5' | -53.2 | NC_005859.1 | + | 1144 | 0.66 | 0.934551 |
Target: 5'- aGGUguauaCAUGCGCUggcauucguuccAGCGUAUCGGgCGAg- -3' miRNA: 3'- -CCG-----GUAUGCGA------------UCGCGUAGUC-GCUau -5' |
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27398 | 5' | -53.2 | NC_005859.1 | + | 112564 | 0.66 | 0.934551 |
Target: 5'- aGGUguauaCAUGCGCUggcauucguuccAGCGUAUCGGgCGAg- -3' miRNA: 3'- -CCG-----GUAUGCGA------------UCGCGUAGUC-GCUau -5' |
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27398 | 5' | -53.2 | NC_005859.1 | + | 94228 | 0.66 | 0.929214 |
Target: 5'- cGCCAgcaGUaAGgGCAUUGGCGAUAg -3' miRNA: 3'- cCGGUaugCGaUCgCGUAGUCGCUAU- -5' |
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27398 | 5' | -53.2 | NC_005859.1 | + | 85147 | 0.67 | 0.905243 |
Target: 5'- uGGCUAUuucgaaGuCUGGUGCuAUCAGCGAg- -3' miRNA: 3'- -CCGGUAug----C-GAUCGCG-UAGUCGCUau -5' |
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27398 | 5' | -53.2 | NC_005859.1 | + | 38255 | 0.67 | 0.898601 |
Target: 5'- cGGCUA-ACGCaGGUGCuuUCGGUGGUGa -3' miRNA: 3'- -CCGGUaUGCGaUCGCGu-AGUCGCUAU- -5' |
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27398 | 5' | -53.2 | NC_005859.1 | + | 68769 | 0.73 | 0.604059 |
Target: 5'- aGCCAUGuuaGCUGGUGUAUCAGCa--- -3' miRNA: 3'- cCGGUAUg--CGAUCGCGUAGUCGcuau -5' |
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27398 | 5' | -53.2 | NC_005859.1 | + | 943 | 1.1 | 0.003131 |
Target: 5'- aGGCCAUACGCUAGCGCAUCAGCGAUAa -3' miRNA: 3'- -CCGGUAUGCGAUCGCGUAGUCGCUAU- -5' |
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27398 | 5' | -53.2 | NC_005859.1 | + | 112362 | 1.1 | 0.003131 |
Target: 5'- aGGCCAUACGCUAGCGCAUCAGCGAUAa -3' miRNA: 3'- -CCGGUAUGCGAUCGCGUAGUCGCUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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