Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2740 | 3' | -45.2 | NC_001491.2 | + | 79722 | 0.67 | 0.999939 |
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Target: 5'- aCACUGAauuauaaGUCUggcuaGCGCGCAGaacGUGCg -3' miRNA: 3'- aGUGAUU-------UAGA-----UGUGCGUCgaaCAUG- -5' |
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| 2740 | 3' | -45.2 | NC_001491.2 | + | 49134 | 0.67 | 0.999918 |
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Target: 5'- cCACUGcAUCUACaaacuggGCGCAGCUagGUucggGCg -3' miRNA: 3'- aGUGAUuUAGAUG-------UGCGUCGAa-CA----UG- -5' |
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| 2740 | 3' | -45.2 | NC_001491.2 | + | 40957 | 0.68 | 0.999861 |
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Target: 5'- gCAC---GUCUGCcCGCAGCUUGc-- -3' miRNA: 3'- aGUGauuUAGAUGuGCGUCGAACaug -5' |
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| 2740 | 3' | -45.2 | NC_001491.2 | + | 142178 | 0.68 | 0.999766 |
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Target: 5'- aUCACcAGGUgUggguacACACGCAGCUUGa-- -3' miRNA: 3'- -AGUGaUUUAgA------UGUGCGUCGAACaug -5' |
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| 2740 | 3' | -45.2 | NC_001491.2 | + | 97055 | 0.7 | 0.998574 |
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Target: 5'- -gGCUAGGUCUGCcaGCGCGGCa----- -3' miRNA: 3'- agUGAUUUAGAUG--UGCGUCGaacaug -5' |
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| 2740 | 3' | -45.2 | NC_001491.2 | + | 135131 | 0.7 | 0.9979 |
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Target: 5'- cCACUGAAUCUcCGCGCugaAGCUgg-GCu -3' miRNA: 3'- aGUGAUUUAGAuGUGCG---UCGAacaUG- -5' |
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| 2740 | 3' | -45.2 | NC_001491.2 | + | 23372 | 0.72 | 0.994121 |
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Target: 5'- cCACgcuGUCUGCAUGguGCUUGc-- -3' miRNA: 3'- aGUGauuUAGAUGUGCguCGAACaug -5' |
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| 2740 | 3' | -45.2 | NC_001491.2 | + | 82543 | 0.72 | 0.990796 |
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Target: 5'- cCAC-AGAUcCUAgGCGCAGCUUGUc- -3' miRNA: 3'- aGUGaUUUA-GAUgUGCGUCGAACAug -5' |
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| 2740 | 3' | -45.2 | NC_001491.2 | + | 62547 | 0.73 | 0.982159 |
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Target: 5'- aCGCUc---CUGCGCGUAGCUauUGUGCu -3' miRNA: 3'- aGUGAuuuaGAUGUGCGUCGA--ACAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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