miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27404 5' -57.5 NC_005869.1 + 300 0.66 0.47404
Target:  5'- uGUGGCACuUCCgCACCACacGUCCg---- -3'
miRNA:   3'- -CGCCGUG-AGG-GUGGUGa-CAGGagacu -5'
27404 5' -57.5 NC_005869.1 + 6277 0.66 0.467867
Target:  5'- gGCGGCggugcuggucgaggaGCUCCCgcaGCCGCUGcugaCC-CUGGg -3'
miRNA:   3'- -CGCCG---------------UGAGGG---UGGUGACa---GGaGACU- -5'
27404 5' -57.5 NC_005869.1 + 17650 0.66 0.463776
Target:  5'- -aGGCcaugGCUCCCACCGCccaacgcGUCaUCUGGc -3'
miRNA:   3'- cgCCG----UGAGGGUGGUGa------CAGgAGACU- -5'
27404 5' -57.5 NC_005869.1 + 7152 0.66 0.443604
Target:  5'- -aGGUACUCCUgAUCGCUGUCC-Ca-- -3'
miRNA:   3'- cgCCGUGAGGG-UGGUGACAGGaGacu -5'
27404 5' -57.5 NC_005869.1 + 23863 0.66 0.443604
Target:  5'- cGCGcGCGCUUCCGCCGC--UCCUg--- -3'
miRNA:   3'- -CGC-CGUGAGGGUGGUGacAGGAgacu -5'
27404 5' -57.5 NC_005869.1 + 25576 0.66 0.443604
Target:  5'- cCGGCAUccugCCCGCCGuCUGcaacgcgcuccCCUCUGAc -3'
miRNA:   3'- cGCCGUGa---GGGUGGU-GACa----------GGAGACU- -5'
27404 5' -57.5 NC_005869.1 + 33801 0.67 0.414299
Target:  5'- cGCGGUACUCuCCACCugUGcgggaauuUCCgccgCUc- -3'
miRNA:   3'- -CGCCGUGAG-GGUGGugAC--------AGGa---GAcu -5'
27404 5' -57.5 NC_005869.1 + 11792 0.67 0.403856
Target:  5'- gGCGGCGCugugcggUCCCugCACg---CUCUGGg -3'
miRNA:   3'- -CGCCGUG-------AGGGugGUGacagGAGACU- -5'
27404 5' -57.5 NC_005869.1 + 23909 0.68 0.368204
Target:  5'- uGCGGC-CUCCC-CCGCg--CCggCUGGg -3'
miRNA:   3'- -CGCCGuGAGGGuGGUGacaGGa-GACU- -5'
27404 5' -57.5 NC_005869.1 + 33299 1.1 0.000251
Target:  5'- aGCGGCACUCCCACCACUGUCCUCUGAc -3'
miRNA:   3'- -CGCCGUGAGGGUGGUGACAGGAGACU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.