Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27405 | 3' | -44.4 | NC_005869.1 | + | 17144 | 0.66 | 0.990856 |
Target: 5'- cGUGGggGugGGCAccGAGCcGGUGGUGc- -3' miRNA: 3'- -CAUCuuUuuUCGU--CUCG-CUACUACua -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 2690 | 0.67 | 0.978106 |
Target: 5'- -cAGAGGGAcGCGGAGCGGgagGAgGAg -3' miRNA: 3'- caUCUUUUUuCGUCUCGCUa--CUaCUa -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 9527 | 0.68 | 0.971793 |
Target: 5'- -gAGggGcAGGCuGAGCGcacUGAUGAUg -3' miRNA: 3'- caUCuuUuUUCGuCUCGCu--ACUACUA- -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 404 | 0.68 | 0.968187 |
Target: 5'- -gAGAAaccGAAAGCGGGGCGA-GAgGAg -3' miRNA: 3'- caUCUU---UUUUCGUCUCGCUaCUaCUa -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 2270 | 0.68 | 0.968187 |
Target: 5'- cUGGAGugccgcGCGGGGCaGGUGGUGAUg -3' miRNA: 3'- cAUCUUuuuu--CGUCUCG-CUACUACUA- -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 17079 | 0.68 | 0.968187 |
Target: 5'- -cGGggGcucGGCAcGGGCGGUGGUGGc -3' miRNA: 3'- caUCuuUuu-UCGU-CUCGCUACUACUa -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 6301 | 0.68 | 0.964266 |
Target: 5'- uUGGAGAGAAGCuuGGCGAUGcugcgGAg -3' miRNA: 3'- cAUCUUUUUUCGucUCGCUACua---CUa -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 4296 | 0.69 | 0.939009 |
Target: 5'- -aGGAAAAAGGUgccguugGGAGUGGUGGUGu- -3' miRNA: 3'- caUCUUUUUUCG-------UCUCGCUACUACua -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 3573 | 0.69 | 0.939009 |
Target: 5'- -aGGAAAAAGGUgccguugGGAGUGGUGGUGu- -3' miRNA: 3'- caUCUUUUUUCG-------UCUCGCUACUACua -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 9942 | 0.72 | 0.823857 |
Target: 5'- cGUGGGcgGAGGUGGcGGCGGUGGUGGc -3' miRNA: 3'- -CAUCUuuUUUCGUC-UCGCUACUACUa -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 15445 | 0.74 | 0.756712 |
Target: 5'- -aGGGAGGGGGCucgucGGAGCGgAUGAUGAUc -3' miRNA: 3'- caUCUUUUUUCG-----UCUCGC-UACUACUA- -5' |
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27405 | 3' | -44.4 | NC_005869.1 | + | 32651 | 1.08 | 0.007668 |
Target: 5'- aGUAGAAAAAAGCAGAGCGAUGAUGAUg -3' miRNA: 3'- -CAUCUUUUUUCGUCUCGCUACUACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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