Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27405 | 5' | -53.2 | NC_005869.1 | + | 26120 | 0.67 | 0.599437 |
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Target: 5'- -gAGCUCAa-CACCGCcgCCCcgUCCa -3' miRNA: 3'- uaUCGAGUcaGUGGUGuaGGGa-AGGg -5' |
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| 27405 | 5' | -53.2 | NC_005869.1 | + | 12276 | 0.67 | 0.599437 |
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Target: 5'- --cGCUCGGg-GCCGCAcaCCUUCCUg -3' miRNA: 3'- uauCGAGUCagUGGUGUagGGAAGGG- -5' |
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| 27405 | 5' | -53.2 | NC_005869.1 | + | 25242 | 0.67 | 0.587813 |
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Target: 5'- -aGGCUCGGUCAgCACGugcUCCaccacaCCCu -3' miRNA: 3'- uaUCGAGUCAGUgGUGU---AGGgaa---GGG- -5' |
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| 27405 | 5' | -53.2 | NC_005869.1 | + | 25454 | 0.68 | 0.519282 |
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Target: 5'- --cGCgCAGgaccUCGCCGCGUUCCUuuUCCCc -3' miRNA: 3'- uauCGaGUC----AGUGGUGUAGGGA--AGGG- -5' |
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| 27405 | 5' | -53.2 | NC_005869.1 | + | 2110 | 0.69 | 0.475374 |
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Target: 5'- uGUGGUcCccuGG-CGCUACAUCCUUUCCCa -3' miRNA: 3'- -UAUCGaG---UCaGUGGUGUAGGGAAGGG- -5' |
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| 27405 | 5' | -53.2 | NC_005869.1 | + | 24454 | 0.7 | 0.403368 |
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Target: 5'- -gAGCUCAGcCGCCGCcuggaagcggCCCUcuucUCCCc -3' miRNA: 3'- uaUCGAGUCaGUGGUGua--------GGGA----AGGG- -5' |
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| 27405 | 5' | -53.2 | NC_005869.1 | + | 32079 | 0.72 | 0.338658 |
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Target: 5'- --cGCUUGGgcacCACCugGUCCCgcaUCCCg -3' miRNA: 3'- uauCGAGUCa---GUGGugUAGGGa--AGGG- -5' |
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| 27405 | 5' | -53.2 | NC_005869.1 | + | 22846 | 0.72 | 0.313327 |
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Target: 5'- -aGGCUCAGcagcgccuccuUCACCAUG-CCCUcgUCCCg -3' miRNA: 3'- uaUCGAGUC-----------AGUGGUGUaGGGA--AGGG- -5' |
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| 27405 | 5' | -53.2 | NC_005869.1 | + | 32685 | 1.1 | 0.000634 |
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Target: 5'- aAUAGCUCAGUCACCACAUCCCUUCCCu -3' miRNA: 3'- -UAUCGAGUCAGUGGUGUAGGGAAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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