Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27406 | 3' | -51.9 | NC_005869.1 | + | 15420 | 0.66 | 0.778103 |
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Target: 5'- cUCCGUGGccGUCACCgggGACC-CGgugGCc -3' miRNA: 3'- uAGGCACUauCAGUGG---CUGGuGUa--CG- -5' |
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| 27406 | 3' | -51.9 | NC_005869.1 | + | 24591 | 0.66 | 0.767392 |
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Target: 5'- -gCCGcGcc-GUCACCGagaccaugcGCCGCAUGCg -3' miRNA: 3'- uaGGCaCuauCAGUGGC---------UGGUGUACG- -5' |
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| 27406 | 3' | -51.9 | NC_005869.1 | + | 17141 | 0.67 | 0.688776 |
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Target: 5'- cUCCGUGggGGUgggCACCGAgCCGgugGUGCa -3' miRNA: 3'- uAGGCACuaUCA---GUGGCU-GGUg--UACG- -5' |
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| 27406 | 3' | -51.9 | NC_005869.1 | + | 25204 | 0.68 | 0.607105 |
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Target: 5'- -aCCGUGG-AGcgCGCCGGCCACGg-- -3' miRNA: 3'- uaGGCACUaUCa-GUGGCUGGUGUacg -5' |
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| 27406 | 3' | -51.9 | NC_005869.1 | + | 16879 | 0.69 | 0.583814 |
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Target: 5'- cUCCGcgcgcUGAUGGgCACCGAgCGgGUGCc -3' miRNA: 3'- uAGGC-----ACUAUCaGUGGCUgGUgUACG- -5' |
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| 27406 | 3' | -51.9 | NC_005869.1 | + | 16877 | 0.7 | 0.515332 |
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Target: 5'- -aCCGUGAUGGaCugC-ACCACcgGCu -3' miRNA: 3'- uaGGCACUAUCaGugGcUGGUGuaCG- -5' |
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| 27406 | 3' | -51.9 | NC_005869.1 | + | 27573 | 0.71 | 0.486648 |
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Target: 5'- cUCCGUGGacgUGGUCACCcuCCACAaccguggcugggccgUGCg -3' miRNA: 3'- uAGGCACU---AUCAGUGGcuGGUGU---------------ACG- -5' |
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| 27406 | 3' | -51.9 | NC_005869.1 | + | 31608 | 1.09 | 0.001099 |
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Target: 5'- cAUCCGUGAUAGUCACCGACCACAUGCc -3' miRNA: 3'- -UAGGCACUAUCAGUGGCUGGUGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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