Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27408 | 3' | -54.5 | NC_005869.1 | + | 15211 | 0.66 | 0.547798 |
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Target: 5'- -aUGUAcGGGGGCGCCCg-CCgCCUGUCc -3' miRNA: 3'- aaAUAU-CCCUUGUGGGgaGG-GGGUAG- -5' |
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| 27408 | 3' | -54.5 | NC_005869.1 | + | 33864 | 0.69 | 0.399025 |
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Target: 5'- ----cAGGGcucCACCCCUUCCCCu-- -3' miRNA: 3'- aaauaUCCCuu-GUGGGGAGGGGGuag -5' |
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| 27408 | 3' | -54.5 | NC_005869.1 | + | 16642 | 0.69 | 0.379929 |
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Target: 5'- --gGUGGGGGGCACCa--CCgCCGUCa -3' miRNA: 3'- aaaUAUCCCUUGUGGggaGGgGGUAG- -5' |
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| 27408 | 3' | -54.5 | NC_005869.1 | + | 21086 | 0.7 | 0.343617 |
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Target: 5'- ----cAGGGc-CGCUCCUCCCCC-UCg -3' miRNA: 3'- aaauaUCCCuuGUGGGGAGGGGGuAG- -5' |
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| 27408 | 3' | -54.5 | NC_005869.1 | + | 30690 | 0.7 | 0.343617 |
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Target: 5'- --cGUGGGGcuGCAUgCCUccaCCCCCAUCc -3' miRNA: 3'- aaaUAUCCCu-UGUGgGGA---GGGGGUAG- -5' |
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| 27408 | 3' | -54.5 | NC_005869.1 | + | 13678 | 0.7 | 0.334937 |
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Target: 5'- ----cAGGGAGCGuCgCCUCCgCCGUCc -3' miRNA: 3'- aaauaUCCCUUGU-GgGGAGGgGGUAG- -5' |
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| 27408 | 3' | -54.5 | NC_005869.1 | + | 23742 | 0.71 | 0.30987 |
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Target: 5'- ----cGGGGGGCGggcCCCCUCcucuuccuCCCCAUCu -3' miRNA: 3'- aaauaUCCCUUGU---GGGGAG--------GGGGUAG- -5' |
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| 27408 | 3' | -54.5 | NC_005869.1 | + | 20875 | 0.71 | 0.30987 |
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Target: 5'- --aGUAGGcGAGCGaguCCCCUCCCuCCcgCu -3' miRNA: 3'- aaaUAUCC-CUUGU---GGGGAGGG-GGuaG- -5' |
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| 27408 | 3' | -54.5 | NC_005869.1 | + | 30722 | 1.06 | 0.000791 |
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Target: 5'- uUUUAUAGGGAACACCCCUCCCCCAUCc -3' miRNA: 3'- -AAAUAUCCCUUGUGGGGAGGGGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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