Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27408 | 5' | -61.1 | NC_005869.1 | + | 19314 | 0.66 | 0.316886 |
Target: 5'- -gGCUGGGcCGGaaGGUCAcCGgguCCUGCAu -3' miRNA: 3'- ggUGACCC-GCC--CCGGU-GCau-GGACGU- -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 5476 | 0.66 | 0.309203 |
Target: 5'- gCCcUUGGcGCggagcuugccGGGGCCGCGgUGCCcGCAa -3' miRNA: 3'- -GGuGACC-CG----------CCCCGGUGC-AUGGaCGU- -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 5692 | 0.66 | 0.309203 |
Target: 5'- gCACgaugaGGGCGGuGGCCagcACGUAgCgGCGg -3' miRNA: 3'- gGUGa----CCCGCC-CCGG---UGCAUgGaCGU- -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 13560 | 0.66 | 0.294265 |
Target: 5'- aCGUUGGGCGGGGaaaaCuuGUACUUGUc -3' miRNA: 3'- gGUGACCCGCCCCg---GugCAUGGACGu -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 10661 | 0.67 | 0.266088 |
Target: 5'- aCCGCaGcGGCucggaGGGGCUGCacUGCCUGCAc -3' miRNA: 3'- -GGUGaC-CCG-----CCCCGGUGc-AUGGACGU- -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 12381 | 0.67 | 0.252838 |
Target: 5'- cCCGCUGGggaGCGcGGG-CACGUGuccucCCUGCu -3' miRNA: 3'- -GGUGACC---CGC-CCCgGUGCAU-----GGACGu -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 7846 | 0.68 | 0.227971 |
Target: 5'- gCGCUGcaGGCGGgcucuggacGGCCACc-ACCUGCAg -3' miRNA: 3'- gGUGAC--CCGCC---------CCGGUGcaUGGACGU- -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 20282 | 0.68 | 0.222086 |
Target: 5'- uCCGCcccucGGUGGGGcCCAgGUACCgcgGCGg -3' miRNA: 3'- -GGUGac---CCGCCCC-GGUgCAUGGa--CGU- -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 15998 | 0.68 | 0.210704 |
Target: 5'- gCCGCUGGaCGGGGCggCGCGUGC--GCGc -3' miRNA: 3'- -GGUGACCcGCCCCG--GUGCAUGgaCGU- -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 9968 | 0.68 | 0.205204 |
Target: 5'- gCgGCggaaGGGUGGGGCCGCGgaggACCccaGCu -3' miRNA: 3'- -GgUGa---CCCGCCCCGGUGCa---UGGa--CGu -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 11231 | 0.69 | 0.194576 |
Target: 5'- gCCGCUGGcUGGuGGaCCugGUgaACCUGCu -3' miRNA: 3'- -GGUGACCcGCC-CC-GGugCA--UGGACGu -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 17471 | 0.69 | 0.189445 |
Target: 5'- gCCGCgGGGCuaGGGGCgGCGUGggUGCu -3' miRNA: 3'- -GGUGaCCCG--CCCCGgUGCAUggACGu -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 12189 | 0.7 | 0.170094 |
Target: 5'- aCGgUGGaGCGGGGCCAgGagagcUACCUGgGg -3' miRNA: 3'- gGUgACC-CGCCCCGGUgC-----AUGGACgU- -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 16675 | 0.71 | 0.132897 |
Target: 5'- gCGCUggagcGGGCGGcGGCCGCcGUGCCccUGCc -3' miRNA: 3'- gGUGA-----CCCGCC-CCGGUG-CAUGG--ACGu -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 26369 | 0.72 | 0.118896 |
Target: 5'- gCGCaGGaGCGGGGCCcCG-GCCUGCc -3' miRNA: 3'- gGUGaCC-CGCCCCGGuGCaUGGACGu -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 208 | 0.72 | 0.103326 |
Target: 5'- uCCGCgGGGCGGGGCCgagcgGCGgaaauuCCcGCAc -3' miRNA: 3'- -GGUGaCCCGCCCCGG-----UGCau----GGaCGU- -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 23876 | 0.74 | 0.079803 |
Target: 5'- gCCGCUccugcacGGGCGGagaGGCCGCGcGCUUGCGg -3' miRNA: 3'- -GGUGA-------CCCGCC---CCGGUGCaUGGACGU- -5' |
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27408 | 5' | -61.1 | NC_005869.1 | + | 30759 | 1.1 | 0.000126 |
Target: 5'- cCCACUGGGCGGGGCCACGUACCUGCAa -3' miRNA: 3'- -GGUGACCCGCCCCGGUGCAUGGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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