Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27410 | 5' | -55.6 | NC_005869.1 | + | 8727 | 0.66 | 0.606159 |
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Target: 5'- gUCGGCaucGUGgGUGGccaccuggcgcAGGAUCUCGGa -3' miRNA: 3'- gAGCUGcu-CACgUACC-----------UCCUGGAGCC- -5' |
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| 27410 | 5' | -55.6 | NC_005869.1 | + | 9464 | 0.66 | 0.561644 |
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Target: 5'- --aGAgGAG-GUagGUGGccAGGGCCUCGGg -3' miRNA: 3'- gagCUgCUCaCG--UACC--UCCUGGAGCC- -5' |
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| 27410 | 5' | -55.6 | NC_005869.1 | + | 10916 | 0.67 | 0.539706 |
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Target: 5'- uUUGAgGGGcGCGUGGAGGAgg-CGGg -3' miRNA: 3'- gAGCUgCUCaCGUACCUCCUggaGCC- -5' |
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| 27410 | 5' | -55.6 | NC_005869.1 | + | 11519 | 0.68 | 0.486282 |
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Target: 5'- -cCGGgGAGgGCGaGGAGGACCU-GGa -3' miRNA: 3'- gaGCUgCUCaCGUaCCUCCUGGAgCC- -5' |
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| 27410 | 5' | -55.6 | NC_005869.1 | + | 19146 | 0.68 | 0.485239 |
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Target: 5'- aCUCuGGCGGGcGCA-GGAGGAUCUgguucucCGGg -3' miRNA: 3'- -GAG-CUGCUCaCGUaCCUCCUGGA-------GCC- -5' |
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| 27410 | 5' | -55.6 | NC_005869.1 | + | 7999 | 0.68 | 0.475893 |
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Target: 5'- -gCGGCGGGUGCccucGGGGGAgCCgauggggCGGa -3' miRNA: 3'- gaGCUGCUCACGua--CCUCCU-GGa------GCC- -5' |
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| 27410 | 5' | -55.6 | NC_005869.1 | + | 4208 | 0.76 | 0.138688 |
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Target: 5'- cCUCGGCGGgGUGCAgguaaagccacUGGAGGGCUgggugCGGg -3' miRNA: 3'- -GAGCUGCU-CACGU-----------ACCUCCUGGa----GCC- -5' |
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| 27410 | 5' | -55.6 | NC_005869.1 | + | 7342 | 0.79 | 0.092794 |
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Target: 5'- aCUUGACGuacuGGUGC-UGGGGGuCCUCGGg -3' miRNA: 3'- -GAGCUGC----UCACGuACCUCCuGGAGCC- -5' |
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| 27410 | 5' | -55.6 | NC_005869.1 | + | 29463 | 1.1 | 0.000527 |
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Target: 5'- cCUCGACGAGUGCAUGGAGGACCUCGGc -3' miRNA: 3'- -GAGCUGCUCACGUACCUCCUGGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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