Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27413 | 3' | -49.4 | NC_005869.1 | + | 26934 | 0.67 | 0.866035 |
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Target: 5'- --aGGAGAgCCAGGGGacgcuuacGACAUCGUCc -3' miRNA: 3'- guaCCUCU-GGUUUCCau------UUGUGGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 26168 | 0.67 | 0.866035 |
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Target: 5'- -cUGGAcGCCGcgcugcAGGUGGugGCCGUCc -3' miRNA: 3'- guACCUcUGGUu-----UCCAUUugUGGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 14992 | 0.66 | 0.873739 |
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Target: 5'- --cGGGGugCAGcGGGUAAccgucaccgacgcGCGgCGCCg -3' miRNA: 3'- guaCCUCugGUU-UCCAUU-------------UGUgGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 18890 | 0.66 | 0.87458 |
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Target: 5'- uGUGG-GACU--GGGUGuccgcacaugcaAGCGCCGUCg -3' miRNA: 3'- gUACCuCUGGuuUCCAU------------UUGUGGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 11606 | 0.66 | 0.87458 |
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Target: 5'- --cGGGGACCGcggcgcguGAGGU-GGCAguccCCGCUg -3' miRNA: 3'- guaCCUCUGGU--------UUCCAuUUGU----GGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 11975 | 0.66 | 0.87458 |
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Target: 5'- uGUGGAGACCuc-GGc---CAuCCGCCc -3' miRNA: 3'- gUACCUCUGGuuuCCauuuGU-GGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 6915 | 0.66 | 0.87458 |
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Target: 5'- gGUGGAGuaAUCGAcGG-AGGCACCGgCg -3' miRNA: 3'- gUACCUC--UGGUUuCCaUUUGUGGCgG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 10288 | 0.66 | 0.890788 |
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Target: 5'- gAUGGGGAggaAGAGGaggGGGC-CCGCCc -3' miRNA: 3'- gUACCUCUgg-UUUCCa--UUUGuGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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