Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27413 | 3' | -49.4 | NC_005869.1 | + | 25900 | 0.69 | 0.755772 |
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Target: 5'- --aGGAGGCCcaGAAGuGcGAGCACUGCg -3' miRNA: 3'- guaCCUCUGG--UUUC-CaUUUGUGGCGg -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 26081 | 0.68 | 0.805081 |
|
Target: 5'- -cUGGAGACCuacaccuGGCACgGCCg -3' miRNA: 3'- guACCUCUGGuuuccauUUGUGgCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 26168 | 0.67 | 0.866035 |
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Target: 5'- -cUGGAcGCCGcgcugcAGGUGGugGCCGUCc -3' miRNA: 3'- guACCUcUGGUu-----UCCAUUugUGGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 26568 | 0.69 | 0.766804 |
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Target: 5'- --cGGAGuaGCCAGccAGGUAgGACACCuCCg -3' miRNA: 3'- guaCCUC--UGGUU--UCCAU-UUGUGGcGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 26934 | 0.67 | 0.866035 |
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Target: 5'- --aGGAGAgCCAGGGGacgcuuacGACAUCGUCc -3' miRNA: 3'- guaCCUCU-GGUUUCCau------UUGUGGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 28354 | 1.12 | 0.001326 |
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Target: 5'- uCAUGGAGACCAAAGGUAAACACCGCCu -3' miRNA: 3'- -GUACCUCUGGUUUCCAUUUGUGGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 28444 | 0.69 | 0.766804 |
|
Target: 5'- aGUGcGAGACCc-AGuGcGAGCGCUGCCg -3' miRNA: 3'- gUAC-CUCUGGuuUC-CaUUUGUGGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 29273 | 0.68 | 0.809177 |
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Target: 5'- --gGGAGACCcagaucugcGAGGgcAACuACCGCa -3' miRNA: 3'- guaCCUCUGGu--------UUCCauUUG-UGGCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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