Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27413 | 3' | -49.4 | NC_005869.1 | + | 16573 | 0.67 | 0.866035 |
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Target: 5'- -cUGGAGggGCUggGGG-AGGCaccggaACCGCCg -3' miRNA: 3'- guACCUC--UGGuuUCCaUUUG------UGGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 4387 | 0.67 | 0.857208 |
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Target: 5'- -uUGGAG-CC-AAGGUGAGgG-CGCCa -3' miRNA: 3'- guACCUCuGGuUUCCAUUUgUgGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 3664 | 0.67 | 0.857208 |
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Target: 5'- -uUGGAG-CC-AAGGUGAGgG-CGCCa -3' miRNA: 3'- guACCUCuGGuUUCCAUUUgUgGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 15843 | 0.67 | 0.829123 |
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Target: 5'- cCGUGGAGGCCAGAGaacUAccaaagcucuucAGC-CCGCUc -3' miRNA: 3'- -GUACCUCUGGUUUCc--AU------------UUGuGGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 29273 | 0.68 | 0.809177 |
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Target: 5'- --gGGAGACCcagaucugcGAGGgcAACuACCGCa -3' miRNA: 3'- guaCCUCUGGu--------UUCCauUUG-UGGCGg -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 19395 | 0.68 | 0.809177 |
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Target: 5'- --gGGAGACCAGcagcgucuGGG-AGGCGgUGCCc -3' miRNA: 3'- guaCCUCUGGUU--------UCCaUUUGUgGCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 4712 | 0.68 | 0.809177 |
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Target: 5'- -uUGGAGACCGgcggcgaggggAAGaGUGAggGCGuuGCCc -3' miRNA: 3'- guACCUCUGGU-----------UUC-CAUU--UGUggCGG- -5' |
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| 27413 | 3' | -49.4 | NC_005869.1 | + | 10288 | 0.66 | 0.890788 |
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Target: 5'- gAUGGGGAggaAGAGGaggGGGC-CCGCCc -3' miRNA: 3'- gUACCUCUgg-UUUCCa--UUUGuGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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